山东省葡萄白腐病菌对氯氟醚菌唑及其他3种三唑类杀菌剂的敏感性

Sensitivity of Coniella vitis in Shandong Province to mefentrifluconazole and three other triazole fungicides

为明确葡萄白腐病菌对氯氟醚菌唑的敏感性,本研究采用菌丝生长速率法测定了山东省6个地市的66株葡萄白腐病菌对氯氟醚菌唑的敏感性,同时通过室内及田间试验比较了氯氟醚菌唑与丙环唑、苯醚甲环唑和戊唑醇3种三唑类杀菌剂对葡萄白腐病的防治效果。结果表明,葡萄白腐病菌群体对氯氟醚菌唑表现为敏感,氯氟醚菌唑对所有菌株的有效抑制中浓度(EC50)在0.0210~1.9464μg/mL之间,平均EC50值为(0.7578±0.4322)μg/mL,其敏感性频率呈连续单峰曲线分布,符合正态分布,因此可将该平均EC50值作为山东地区葡萄白腐病菌对氯氟醚菌唑的敏感基线。丙环唑、苯醚甲环唑和戊唑醇对随机选择的31株葡萄白腐病菌的平均EC50值分别为(1.1667±0.4456)、(2.1577±0.5007)和(5.8187±1.5538)μg/mL。结果表明,氯氟醚菌唑对葡萄白腐病菌的活性与丙环唑、苯醚甲环唑相当,优于戊唑醇,该结果可为田间葡萄白腐病菌抗药性监测及防控提供依据。

In order to clarify the sensitivity of Coniella vitis to mefentrifluconazole,the sensitivity of 66 C.vitis isolates from 6 cities in Shandong Province to mefentrifluconazole was determined by the mycelial growth rate method.Additionally,the indoor and field control efficacy of mefentrifluconazole and three triazole fungicides, propiconazole, difenoconazole, and tebuconazole was compared. The results showed the population of C. vitis exhibited sensitivity to mefentrifluconazole, with effective inhibition concentrations (EC50) ranging from 0.0210 to 1.9464 μg/mL and an average value of (0.7578 ± 0.4322) μg/mL. The sensitivity frequency is distributed in a continuous unimodal curve, conforming to a normal distribution. Therefore, the average EC50 value can be considered as the baseline sensitivity of C. vitis in Shandong Province to mefentrifluconazole. The average EC50 values of propiconazole, difenoconazole and tebuconazole against 31 randomly selected isolates were (1.1667 ± 0.4456), (2.1577 ± 0.5007) and (5.8187 ± 1.5538) μg/mL, respectively. The experiments showed the activity of mefentrifluconazole against C. vitis was comparable to that of propiconazole and difenoconazole, and better than that of tebuconazole. These findings provide a basis for monitoring pesticide resistance and effective control of C. vitis in fields.