四个长阶花品种叶片对高温胁迫形态及生理响应

Leaf Morphology and Physiological Response of Four Hebes Varieties Under High Temperature Stress

以4个长阶花品种为试材,利用人工气候培养箱模拟高温胁迫,研究不同高温胁迫对4个长阶花品种的形态、色素含量及生理指标的影响,以期筛选出抗热能力较强的长阶花品种。结果表明:通过表型分析,4个长阶花品种生长指标中的叶面积增量显著减少。当胁迫达到35℃时,‘银色卡特琳娜’热害严重;当温度达到40℃时,各品种长阶花存活率骤降为0%。通过色素含量及生理指标分析,长阶花为阴生植物。随着高温胁迫程度的加强,4个长阶花品种叶片色素含量整体呈现出逐渐降低的趋势,相对电导率值、可溶性蛋白质含量、脯氨酸含量及过氧化物酶(POD)活性整体呈现逐渐增加的趋势,过氧化氢酶(CAT)活性除‘珍娜’呈持续上升趋势外,其他品种呈先上升后下降的趋势。‘珍娜’品种在高温下存活率较高,有益物质积累最多且膜透性更稳定。因此初步判定,‘珍娜’品种耐热性稍好,其他3个品种长阶花耐热性均较差。

Taking four hebe varieties as test materials,using artificial climate incubator to simulate high temperature stress to study the morphology,pigment content and physiological indexes of them,in order to select hebe varieties with strong heat resistance.The results showed that the leaf area increment in the growth index of hebe varieties was significantly reduced by phenotype analysis.When the stress reached 35℃,the‘Silver-KARINA’was severe;when the temperature reached 40℃,the survival rate of each variety dropped to 0%.Through the analysis of pigment content and physiological indicators,hebe were shady plants.With the strengthening of high temperature stress degree,hebe varieties leaf pigment content showed a trend of gradually decreased,the relative conductivity value,soluble protein content,proline content and POD activity were gradually increasing trend,CAT activity except‘Genna’continued rising trend,other varieties rose first.‘Genna’variety had a high survival rate at high temperature,the most accumulation of beneficial substances and more stable membrane permeability.Therefore,it was preliminarily determined that the heat resistance of‘Genna’varieties was slightly better,while the heat resistance of the other three varieties was poor.