葛根素调控Wnt/β-catenin途径影响大鼠逼尿肌舒缩活动

Puerarin affects the relaxation and contraction of detrusor muscle in rats by regulating Wnt/β-catenin pathway

[目的]分析葛根素对动物模型逼尿肌舒缩活动和Wnt/β-catenin途径的影响。[方法]选择50只大鼠随机分为对照组、模型组、葛根素10 mg组、葛根素20 mg组、葛根素30 mg组;模型组和葛根素各组大鼠建立骶上脊髓损伤大鼠模型;后依照分组给予葛根素和生理盐水干预;1个月后检测各组大鼠尿流动力学,并分析组织中α1G、c-kit、Wnt1和β-catenin蛋白及mRNA水平。[结果]对照组、模型组、葛根素10 mg组、葛根素20 mg组和葛根素30 mg组大鼠MBC、BLPP、CF、α1G、c-kit、Wnt1和β-catenin蛋白及mRNA水平存明显差异(P<0.05),其中葛根素各组大鼠MBC、BLPP、CFF、α1G、c-kit、Wnt1和β-catenin蛋白及mRNA水平与模型组存明显差异(P<0.05),葛根素各组中葛根素30 mg组大鼠MBC、Wnt1和β-catenin蛋白及mRNA水平最高,BLPP、CF、α1G、c-kit蛋白及mRNA水平最低(P<0.05)。[结论]葛根素可有效激活Wnt/β-catenin途径并改善动物模型逼尿肌舒缩活动。

[Objective]To analyze the effect of puerarin on the detrusor contraction and Wnt/β-catenin pathway in animal models.[Method]Fifty rats were randomly divided into a control group,a model group,a puerarin 10 mg group,a puerarin 20 mg group,and a puerarin 30 mg group;the model group and the puerarin group rats established a model of suprasacral spi-nal cord injury Afer the intervention of puerarin and saline according to grouping;one month later,the urodynamics of rats in each group were detected,and the levels of alG,c-kit,Wntl and β-catenin in the tissue were analyzed.[Result] There were significant differences in the protein and mRNA levels of MBC,BLPP,CF,αlG,c-kit,Wntl and β-catenin in the control group,model group,puerarin 10 mg group,puerarin 20 mg group and puerarin 30 mg group(P<0.05),and the protein and mRNA levels of MBC,BLPP,CFF,alG,c-kit,Wntl and β-catenin in the puerarin group were significantly different from the model group(P<0.05),among the puerarin groups,the puerarin 30 mg group had the highest protein and mRNA levels of MBC,Wntl and β-catenin,and the lowest protein and mRNA levels of BLPP,CF,αlG and c-kit(P<O.05).[Conclusion]Puerarin can effectively activate Wnt/β-catenin pathway and improve detrusor muscle contraction and contraction in animal models.