癫痫患儿血清25-羟维生素D及IgG、IgM、IgA的变化及意义

Changes and significance of serum 25-hydroxyvitamin D,IgG,IgM and IgA in children with epilepsy

目的探讨癫痫患儿血清25-羟维生素D及体液免疫因子免疫球蛋白(immunoglobulin,Ig)G、IgM、IgA的变化及意义。方法选取2019年12月至2020年12月于滨州医学院附属医院就诊的初诊癫痫患儿作为病例组(54例),同期选取来我院儿童保健科就诊的健康儿童作为对照组(50例)。酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测25-羟维生素D水平;免疫比浊法检测IgG、IgA、IgM表达。6个月后再次抽取静脉血,检测25-羟维生素D及IgG、IgA、IgM表达水平,对比分析初诊时及6个月后25-羟维生素D、IgG、IgM、IgA的变化。结果与对照组相比,初诊时及6个月后,病例组患儿血清25-羟维生素D水平显著降低,差异有统计学意义(t=2.45、8.15,P值均<0.05);与初诊时相比,治疗后6个月后病例组患儿25-羟维生素D水平显著下降,差异具有统计学意义[ng/mL:(21.46±1.14)比(19.60±1.60),t=9.18,P<0.05]。与对照组相比,初诊时病例组患儿IgG、IgM、IgA水平明显降低,差异有统计学意义(t=2.78、4.82、9.57,P值均<0.05);与初诊时相比,治疗6个月后,病例组患儿IgG、IgM、IgA水平明显下降,差异具有统计学意义[g/L:(562.65±10.54)比(588.17±16.05),(70.38±0.98)比(71.55±1.75),(24.00±1.57)比(27.40±1.34),t=10.66、5.44、12.07,P值均<0.05]。结论初诊癫痫患儿即存在维生素D缺乏和体液免疫功能受损,维生素D缺乏及免疫功能变化可能参与癫痫的发生发展。

Objective Exploring the changes and significance of serum 25 hydroxyvitamin D and humoral immune factors immunoglobulin(Ig)G,IgM,and IgA in children with epilepsy.Method Total of 54 newly diagnosed children with epilepsy who visited the Affiliated Hospital of Binzhou Medical College from December 2019 to December 2020 were selected as the patient group,while 50 healthy children who visited the Children's Health Department of our hospital during the same period were selected as the control group.Enzyme linked immunosorbent assay(ELISA)was used to detect the level of 25 hydroxyvitamin D.Immunoassay was used to detect the expression of IgG,IgA and IgM.Six months later,venous blood was drawn again to detect the expression levels of 25 hydroxyvitamin D,IgG,IgA and IgM.The changes in 25 hydroxyvitamin D,IgG,IgM,and IgA were analyzed and compared at initial diagnosis and six months later.Results Compared with the control group,the serum levels of 25 hydroxyvitamin D in the patient group were significantly lower at initial diagnosis and 6 months later(t=2.45,8.15,both P values<0.05).Compared with the initial diagnosis,the patient group showed a significant decrease in 25 hydroxyvitamin D levels after 6 months of treatment[ng/mL:(21.46±1.14)vs(19.60±1.60),t=9.18,P<0.05].Compared with the control group,the levels of IgG,IgM and IgA in the patient group decreased significantly at initial diagnosis(t=2.78,4.82,9.57,all P values<0.05).Compared with the initial diagnosis,after 6 months of treatment,the levels of IgG,IgM and IgA in the patient group were significantly decreased[g/L:(562.65±10.54)vs(588.17±16.05),(70.38±0.98)vs(71.55±1.75),(24.00±1.57)vs(27.40±1.34),t=10.66,5.44,12.07,all P values<0.05].Conclusion Children with newly diagnosed epilepsy have vitamin D deficiency and impaired humoral immune function.Vitamin D deficiency and changes in immune function may be involved in the occurrence and development of epilepsy.