增生白玉芦汤对大鼠放射性口腔黏膜炎的治疗作用

Effects of ZengshengBaiyulu decoction on radiation-induced oral mucositis in rats

目的 探讨增生白玉芦汤对大鼠放射性口腔黏膜炎的防治作用及其机制。方法 取SPF级♂SD大鼠50只,除空白组10只外,所有大鼠头颈部接受25Gy X线放射照射1次,次日ig给药治疗10 d,每日1次,记录各组大鼠的一般情况、体重和颊黏膜变化;治疗结束后,收集心脏血并处死大鼠,收集颊黏膜组织用于相关炎性因子、蛋白、基因和病理检测。结果 增生白玉芦汤组大鼠的一般情况较好,体质量下降程度小,口腔黏膜炎的程度较轻;白细胞介素-1β(IL-1β)、IL-6、IL-10、肿瘤坏死因子-α(TNF-α)的水平及颊黏膜组织中IL-1β、TNF-α、磷酸化核因子κB(NF-κB)抑制蛋白α、磷酸化NF-κB的表达降低,但黏膜组织中表皮生长因子、血管内皮生长因子、碱性成纤维细胞生长因子的含量升高。结论 增生白玉芦汤能有效改善减轻大鼠口腔黏膜的炎性症状,减轻及修复颊黏膜损伤,其机制可能与抑制NF-κB信号通路及促进生长因子有关。

OBJECTIVE To investigate the preventive effect and mechanism of Zengsheng Baiyulu decoction(ZBD)on radiation-induced oral mucositis in rats.METHODS In this study,50 male SD rats of SPF grade were selected.All rats received 25Gy X-ray radiation once on their head and neck except for 10 rats in the blank group.The next day,the rats were treated by intragastric administration of drugs for 10 days,once daily.The general condition,body weight and changes in buccal mucosa were recorded.After treatment,cardiac blood was collected and killed.Buccal mucosa tissues were collected for the detection of relevant inflammatory factors,proteins,genes and pathology.After treatment,cardiac blood was collected and killed,and buccal mucosa tissues were collected for the detection of relevant inflammatory factors,proteins,genes and pathology.RESULTS The rats in the ZBD group were generally in good condition,with minimal reduction in body mass and a mild degree of oral mucositis.The levels of IL-1β,IL-6,IL-10 and TNF-α and the expressions of IL-1β and TNF-α,P-IKBαand P-NF-κB in buccal mucosa were upregulated,while the levels of ECF,VEGF and bFGF were increased in buccal mucosa.CONCLUSION ZBD has been demonstrsted to be an effective treatment for inflammatory symptoms of oral mucosa as well as for the repair of buccal mucosa in irradiated rats.The mechanism of action may be related to the inhibition of the NF-κB signaling pathway and the promotion of growth factors.