基于代谢组学研究金钗石斛调节脂质代谢的作用

Regulation of lipid metabolism by Dendrobium nobile Lindl.based on metabolomics

目的基于代谢组学研究金钗石斛对脂质代谢的调节作用。方法将24只大鼠随机分为空白对照组、金钗石斛给药组(0.25 g/kg),每6 h灌胃1次,连续5 d,收集大鼠血浆和肝脏。采用UPLC-MS对血浆样本进行代谢组学研究。利用主成分分析、聚类分析、KEGG通路富集分析等方法,对金钗石斛给药组与空白对照组的代谢组学数据进行分析,比较差异代谢物。根据代谢组学结果,采用RT-qPCR检测金钗石斛对Cyp7a1、Cyp7b1、Cyp8b1、Cyp27a1、Cyp3a1和Lpin1等脂质代谢相关基因表达的影响。结果与空白对照组相比,金钗石斛给药组血浆样本内源性代谢物整体轮廓变化明显,其中1448个代谢物的变化显著(P<0.05),641个代谢物的差异倍数≥2。KEGG通路富集结果显示,前5条通路分别为Metabolic pathways、Biosynthesis of antibiotics、ABC transporters、Biosynthesis of amino acids、Protein digestion and absorption。RT-qPCR结果表明,金钗石斛可以上调Cyp27a1和Cyp3a1基因的表达(P<0.05),下调Lpin1基因的表达(P<0.05)。结论金钗石斛能对脂质代谢进行调节,为其进一步的开发与利用提供了科学依据。

Objective To determine the effects of Dendrobium nobile Lindl.on the regulation of lipid metabolism based on metabolomics.Methods Twenty-four rats were randomly divided into the normal control and D.nobile treatment(0.25 g/kg)groups.Rats were intragastrically administered their assigned treatment once every 6 h for 5 d.After the last treatment,the plasma and liver of the animals were collected.Metabolomics were evaluated using UPLC-MS.Principal component analysis(PCA),cluster analysis,and KEGG pathway analysis were performed to assess the metabolomics data.Compared with the normal control group,different metabolites were identified in the D.nobile treatment group.The expression levels of lipid metabolism-related genes were validated using quantitative real-time PCR.Results Obvious differences were found between the normal control group and D.nobile treatment group,with significant differences obtained for 1448 metabolites(P<0.05)and a fold change greater than 2 recorded for 641 metabolites.Based on KEGG pathway analysis,metabolic pathways,biosynthesis of antibiotics,ABC transporters,biosynthesis of amino acids,protein digestion and absorption were among the top five pathways.D.nobile could significantly upregulate the gene expression levels of Cyp27a1 and Cyp3a1(P<0.05)and significantly downregulate the gene expression of Lpin1(P<0.05).Conclusion Overall,D.nobile could regulate lipid metabolism.Such findings provide a scientific basis for the further development and utilization of D.nobile.