副溶血性弧菌快速定量检测方法研究进展

Research progress in the rapid quantitative determination of Vibrio parahaemolyticus

副溶血性弧菌(VP)是我国沿海地区食物中毒最常见的病原菌之一,引发的食品安全事件逐年上升。目前定量检测食品中副溶血性弧菌的国家标准方法为最大可能数法,该方法检测时限长且操作步骤繁琐,不能满足快速定量检测的需求。随着现代生物技术的发展,新的定量检测方法不断涌现,研发了多种快速灵敏的副溶血性弧菌定量检测方法。本文综述了近年来生物技术领域常见的定量方法在副溶血性弧菌快速定量检测中的研究现状及应用进展,充分了解各检测方法的特点,以期为食品中副溶血性弧菌的快速精准定量检测研究提供理论参考及发展方向。

Vibrio parahaemolyticus(VP)is one of the most common pathogens that cause food poisoning in coastal areas of China.In fact,the food safety incidents of VP have been increasing on a yearly basis.The current national standard method for quantitatively detecting VP in food is the most probable number method.This method is time-consuming and tedious,and cannot be used to achieve rapid quantitative detection.Owing to modern biotechnology,new quantitative methods have been generated.In fact,a series of rapid and sensitive methods have been developed to quantify VP.This review seeks to discuss recent research and application progress of rapid methods for quantifying VP using biotechnology methods,and highlight the characteristics of each rapid method to provide a theoretical reference and development direction for the rapid and accurate quantitation of VP in food.