连花清瘟颗粒对慢性鼻窦炎不伴鼻息肉患者鼻黏膜功能的影响

The influence of lianhuaqingwen granules on the function of nasal mucosa in chronic rhinosinusitis without nasal polyps

目的慢性鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSsNP)是一种复杂的疾病,连花清瘟颗粒对该病有一定的治疗作用,但其机制尚不明确。本研究旨在探讨该药对CRSsNP患者鼻黏膜功能的影响。方法招募CRSsNP患者72例并进行连花清瘟颗粒治疗,时间为4周,在治疗前后分别检测患者鼻黏液纤毛清除功能(nasal mucociliary clearance,NMC)和鼻内一氧化氮(nitric oxide,NO)浓度,获取患者治疗前后鼻黏膜标本,进行HE染色,并用酶联免疫吸附试验检测其中环磷酸腺苷(cyclic adenosine monophosphate,cAMP)、三磷酸肌醇(inositol trisphosphate,IP3)、白介素(interleukin,IL)-8和IL-13的含量。结果70例CRSsNP患者完成了研究,NMC在连花清瘟颗粒治疗后显著缩短,治疗后的鼻内NO浓度较治疗前显著升高,组织学检查显示鼻黏膜上皮细胞的纤毛在治疗后排列整齐,而且连花清瘟颗粒治疗后,组织中cAMP和IP3的含量显著,IL-8和IL-13的含量显著减少。结论连花清瘟颗粒可以促进CRSsNP患者鼻黏膜功能的修复。

Objective Chronic rhinosinusitis without nasal polyps(CRSsNP)is a complicated disease.Lianhuaqingwen granules have a role in the treatment of CRSsNP.However,the mechanisms need to be clearly elucidated.The study aimed to explore the influence of lianhuaqingwen on the function of nasal mucosa in CRSsNP.Methods Seventy-two CRSsNP patients were enrolled and received lianhuaqingwen granules treatment for four weeks.Nasal mucociliary clearance(NMC)and nitric oxide(NO)were examined before and after the treatment.Nasal biopsy samples were obtained and stained using hematoxylin-eosin.Then,concentrations of cyclic adenosine monophosphate(cAMP),inositol trisphosphate(IP3),interleukin(IL)-8 and IL-13 were evaluated with enzyme-linked immunosorbent assay.Results Seventy CRSsNP patients completed the study.NMC was reduced significantly after lianhuaqingwen granules therapy,notwithstanding,the content of nasal NO was increased.Histological analysis indicated that nasal epithelial cell cilia were arranged regularly after the treatment.Furthermore,concentrations of cAMP and IP3 were elevated,and levels of IL-8 and IL-13 were decreased in the nasal samples after lianhuaqingwen granules administration.Conclusion Lianhuaqingwen granules may restore the functions of nasal mucosa in CRSsNP patients.