cudraflavone B对MRSA抗菌作用及机制初步研究

Antibacterial Effect and Mechanism of Flavonoid Compound Cudraflavone B on MRSA

考察黄酮类化合物cudraflavone B对耐甲氧西林金黄色葡萄球菌(MRSA1001)的抑菌效果和作用机制.采取微量肉汤稀释法测定cudraflavone B的最低抑菌浓度及最低杀菌浓度,并绘制其对MRSA1001的时间—杀菌曲线,进行溶血活性和细胞毒性试验评估其安全性,最后通过扫描电子显微镜观察经cudraflavone B处理后MRSA1001细胞的形态变化.结果表明,cudraflavone B对革兰氏阳性菌表现出明显的抑制作用,杀菌作用较弱,并对MRSA1001表现出一定的浓度依赖性杀菌活性;对人胚胎肾细胞未显示出明显的细胞毒性,对兔红细胞也表现出较低溶血活性,表明其安全性较好;在扫描电子显微镜下观察到,经cudraflavone B处理后的细菌细胞呈现出塌陷并伴有细胞内物质渗出的现象,表明其作用机制可能是通过破坏细菌细胞膜,造成细菌死亡从而达到抗菌效果.cudraflavone B可作为抗菌药物继续发掘其价值.

To investigate the antibacterial effect and mechanism of flavonoid codpound cudraflavone B on methicillin-resistant staphylococcus aureus(MRSA1001),the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of cudraflavone B were determined by using the micro broth dilution method,and its time bactericidal curve against MRSA1001 was plotted.Its safety was evaluated through hemolytic activity and cytotoxicity tests.Finally,the morphological changes of MRSA1001 after treatment were observed by scanning electron microscopy.The results showed that cudraflavone B exhibited a significant inhibitory effect on Gram-positive bacteria,weak bactericidal effect,and showed a certain concentration dependent bactericidal activity against MRSA1001.No significant cytotoxicity was observed on HEK293 cells,and low hemolytic activity was also observed on rabbit red blood cells,which indicated high safety.Under scanning electron microscopy,bacterial cells treated with cudraflavone B showed collapse accompanied by liquid exudation,indicating that its mechanism of action might be achieved by disrupting the bacterial cell membrane,and thereby achieving antibacterial effects.In summary,cudraflavone B can continue to be explored for its value as an antibacterial drug.