家蚕响应微孢子虫感染的可变剪接事件分析

Analysis on alternative splicing events in silkworm's response to Nosema bombycis infection

【目的】家蚕微孢子虫(Nosema bombycis)感染引发的微粒子病是影响蚕业发展的重要病害。为了揭示可变剪接(AS)基因在家蚕响应微孢子虫感染中的作用,对其中肠内的AS事件进行分析。【方法】利用Illumina Hiseq TM 4000测序平台,对健康家蚕(对照组)和被微孢子虫感染的家蚕中肠组织进行转录组测序。使用rMATS软件对样品中的AS事件与差异剪接基因(DSGs)进行鉴定,并对DSGs进行GO富集和KEGG信号通路分析。【结果】在对照组和感染组中分别检测到5346个和4992个AS事件,两组的AS事件类型都以外显子跳跃(SE)事件为主,差异分析发现了25个显著的DSGs。GO富集分析表明,DSGs主要富集在21个条目,其中以细胞过程、物质代谢、结合、催化活性的基因数量较多;KEGG信号通路分析显示,DSGs富集数较多的是信号传导、免疫系统、多糖生物合成与降解、能量代谢等通路。【结论】家蚕中肠组织存在大量AS事件,在微孢子虫感染过程中存在25个DSGs,其功能涉及新陈代谢和细胞免疫等。

【Objective】The pebrine disease of silkworm caused by the infection of Nosema bombycis(Nb)is a severe threat to sericultural industry.In order to reveal the role of genes participating in alternative splicing(AS)events during silkworm's response to Nb infection,the AS events in the midgut were analyzed.【Method】Transcriptome sequencing was performed on midgut tissues from Nb-infected silkworms(NB)and uninfected groups(CK)with Illumina Hiseq TM 4000 platform.AS events and differentially spliced genes(DSGs)were identified via rMATS software,followed by GO enrichment and KEGG signaling pathway analysis on DSGs.【Result】A total of 5346 and 4992 AS events were detected in the NB and CK groups,respectively,in which skipped exon(SE)events were dominant in both groups.A total of 25 significant DSGs were identified between CK and NB.GO enrichment analysis showed that the DSGs were significantly enriched in 21 items,such as cellular process,substance metabolism,binding and catalytic activity;while KEGG pathway analysis showed the DSGs were mostly involved in signal transduction,immune system,polysaccharides biosynthesis and metabolism,and energy metabolism pathways.【Conclusion】There were numerous AS events in the midgut tissue of silkworms,and the Nb infection caused 25 DSGs which were relevant to metabolism and cellular immunity.