甜椒幼苗低温胁迫的生理响应与转录组分析

Physiological Response and Transcriptome Analysis of Sweet Pepper Seedlings Under Low Temperature Stress

为了阐明甜椒响应低温胁迫的分子机制,对低温胁迫时甜椒幼苗的MDA含量、SOD活性以及光合作用相关参数进行了测定,并结合转录组测序分析。结果表明:低温胁迫3 d时甜椒MDA含量显著升高,SOD活性显著降低,光合相关参数显著下降;与低温处理前相比,转录组测序分析结果显示检测出差异基因主要涉及酶系统、光合作用、信号传导以及相关转录因子,其中SOD相关基因CaSOD呈示上调表达,光合作用关键基因CaCP4与CaHCR的表达量则显著下调。研究结果为深入解析甜椒幼苗响应低温胁迫的分子机制及甜椒抗低温育种奠定了基础。

In order to clarify the molecular mechanism of sweet pepper in response to low temperature stress,the MDA content,SOD activity of sweet pepper seedlings and the relative parameters of photosynthesis under the low temperature stress were determined,and the transcriptome was sequenced and analyzed.The results showed that the content of MDA in sweet pepper increased significantly,the activity of SOD decreased significantly,and the photosynthetic relative parameters decreased significantly under the low temperature stress for 3 days.Compared with those before the treatment of low temperature,the transcriptome sequencing analysis showed that the differentially expressed genes were mainly involved in the enzyme system,photosynthesis,signal transduction and related transcription factors.Among them,the expression of SOD-related gene CaSOD was up-regulated,and the expression of photosynthesis key genes CaCP4 and CaHCR was significantly down-regulated.The results laid a foundation for the further analysis of the molecular mechanism of sweet pepper seedlings in response to the low temperature stress and the anti-low temperature breeding of sweet pepper.