结直肠癌组织中ACOX1、DUSP14蛋白表达变化及临床意义

Expression changes and clinical significance of ACOX1 and DUSP14 in colorectal cancer tissues

目的探讨结直肠癌组织中酰基辅酶A氧化酶1(ACOX1)、双特异性磷酸酶14(DUSP14)蛋白表达变化及临床意义。方法选择手术治疗的98例CRC患者,术中取癌和癌旁组织(距肿瘤边缘>2 cm)。用免疫组化染色法检测组织中ACOX1、DUSP14蛋白,用Spearman秩相关分析CRC癌组织中ACOX1与DUSP14蛋白表达的相关性,以及二者表达与临床病理参数的关系;对患者进行随访,记录其生存状况,Cox回归模型分析CRC预后的影响因素。结果CRC癌组织、癌旁组织中ACOX1蛋白阳性表达率分别为24.49%(24/98)、84.69%(83/98),二者比较差异有统计学意义(P<0.05)。CRC癌组织、癌旁组织中DUSP14蛋白阳性表达率分别为79.59%(78/98)、12.24%(12/98),二者比较差异有统计学意义(P<0.05)。CRC癌组织中ACOX1与DUSP14蛋白表达呈负相关(rs=-0.792,P<0.05)。与TNM分期Ⅰ、Ⅱ期,高中分化及无淋巴结转移的CRC组织比较,TNM分期Ⅲ期、低分化程度及淋巴结转移的CRC组织中ACOX1蛋白阳性表达率低,DUSP14蛋白阳性表达率高(P均<0.05)。CRC患者随访期间死亡42例,3年总生存率为57.14%(56/98)。ACOX1蛋白表达阳性与阴性患者3年生存率分别为87.50%(21/24)、47.30%(35/74),二者比较差异有统计学意义(P<0.05);DUSP14蛋白表达阳性与阴性患者3年生存率分别为48.72%(38/78)、90.00%(18/20),二者比较差异有统计学意义(P<0.05)。ACOX1蛋白表达阴性、DUSP14蛋白表达阳性、TNM分期Ⅲ期及淋巴结转移为CRC患者预后的危险因素(P均<0.05)。结论CRC中ACOX1低表达、DUSP14高表达,二者表达变化与肿瘤发展及预后有关。

Objective To investigate the expression changes of acyl-CoA oxidase 1(ACOX1)and bispecific phosphatase 14(DUSP14)in the colorectal cancer(CRC)tissues and their clinical significance.Methods Ninety-eight CRC patients who underwent surgical treatment were selected,and the cancer and adjacent tissues(more than 2 cm away from the tumor edge)were taken during surgery.Immunohistochemistry was used to detect the expression of ACOX1 and DUSP14 proteins in the tissues.Spearman rank correlation analysis was used to analyze the correlation between the expression of ACOX1 and DUSP14 proteins in CRC cancer tissues,as well as the relationship between their expression levels and the clinical pathological parameters;the patients were followed up,and we recorded their survival status,and used the Cox regression model to analyze the influencing factors for the prognosis of CRC.Results The positive expression rates of ACOX1 protein in the CRC cancer tissues and adjacent cancer tissues were 24.49%(24/98)and 84.69%(83/98),respectively,and the difference was statistically significant(P<0.05).The positive expression rates of DUSP14 protein in the CRC cancer tissues and adjacent cancer tissues were 79.59%(78/98)and 12.24%(12/98),respectively,and the difference was statistically significant(P<0.05).The expression of ACOX1 and DUSP14 proteins in CRC cancer tissues was negatively correlated(rs=–0.792,P<0.05).Compared with the CRC tissues at TNM stages I and II,with high and middle differentiation,and without lymph node metastasis,the positive expression rates of ACOX1 protein in the CRC tissues at TNM stage III,with low degree of differentiation,and with lymph node metastasis were lower,and the positive expression rates of DUSP14 protein were higher(all P<0.05).During the follow-up period of CRC patients,42 cases died,and the 3-year overall survival rate was 57.14%(56/98).The 3-year survival rates of patients with positive and negative expression of ACOX1 protein were 87.50%(21/24)and 47.30%(35/74),respectively,with statistically significant differences between the groups(P<0.05).The 3-year survival rates of patients with positive and negative expression of DUSP14 protein were 48.72%(38/78)and 90.00%(18/20),respectively,with statistically significant differences between the groups(P<0.05).The negative expression of ACOX1 protein,the positive expression of DUSP14 protein,the TNM stage III and lymph node metastasis were risk factors for the prognosis of CRC patients(all P<0.05).Conclusion In CRC,ACOX1 is low expressed and DUSP14 is highly expressed,and the expression changes of the two are related to tumor development and prognosis.