纳米硒对甜菜叶片转录组的影响

Effects of Nano-selenium on the Transcriptome of Sugar Beet Leaves

为揭示喷施外源硒对甜菜响应的分子机制,采用糖甜菜品系HD802种子作为供试材料进行水培试验,纳米硒浓度分别设置为0(喷施清水,对照),20,50,80,100,150,200 mg/L,待真叶长至8片叶时均匀地喷施纳米硒溶液,24 h后取样进行超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量测定。经数据分析,选取50,150 mg/L的纳米硒处理叶片送检,进行转录组测定,分析差异表达基因和显著富集通路。结果表明,纳米硒50 mg/L处理对甜菜叶片生长具有促进作用,而150 mg/L处理对甜菜叶片具有破坏效果。鉴定出9 161个DEGs,其中,上调表达3 717个、下调表达5 444个;GO功能富集主要富集在信号转导、细胞通讯、膜的组成部分、膜的固有成分、初级代谢过程、有机物代谢过程和生物过程等;KEGG代谢途径主要包括植物病原互作、植物激素信号转导等途径;转录因子分析共涉及AP2、zf-Dof、HLH、WRKY、HSF_DNA-bind、NAM、zf-BED、Homeobox等11个家族。研究找到了硒处理对甜菜幼苗生长发育有促进作用的适宜浓度,初步筛选出甜菜外源硒响应的相关基因。

In order to reveal the molecular mechanism of the response to exogenous selenium of sugar beet,seeds of the sugar beet line HD802 were used as test materials for hydroponic experiments.The concentrations of nano-selenium were set to 0(water spray,control),20,50,80,100,150,200 mg/L,respectively.When the plants grew to 8 true leaves stage,nano-selenium solution was evenly sprayed.After 24 h,the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and the content of malondialdehyde(MDA)were measured.After data analysis,50,150 mg/L nano-selenium treated leaves were selected for examination.RNA-Seq was performed to analyze the differentially expressed genes and significant enrichment pathways.The results showed that 50 mg/L nano-selenium treatment had promoting effects on the growth of sugar beet leaves,while 150 mg/L treatment had destructive effects on sugar beet leaves.A total of 9161 DEGs were identified,of which 3717 were up-regulated and 5444 were down-regulated.GO functional enrichment was mainly enriched in processes such as signal transduction,cell communication,integral component of membrane,intrinsic component of membrane,primary metabolic process,organic substance metabolic process,and biological process.The metabolic pathways of KEGG mainly included plant pathogen interaction,plant hormone signal transduction,and other pathways.Transcription factor analysis involved 11 families,including AP2,zf-Dof,HLH,WRKY,HSF_DNA-bind,NAM,zf-BED and Homeobox.It found the optimal concentration for selenium treatment to promote the growth and development of sugar beet seedlings,and preliminarily screened the relevant genes responsive to exogenous selenium in sugar beet.