炎性肺上皮细胞模型中右美托咪定与JAK2/STAT3信号通路的相关性

Correlation Between Dexmedetomidine and JAK2/STAT3 Signaling Pathway in Inflammatory Lung Epithelial Cell Model

目的研究右美托咪定(DEX)在白细胞介素-6(IL-6)诱导的炎性肺上皮细胞模型中对Janus激酶2/信号转导子和转录激活子3(JAK2/STAT3)信号通路的作用及机制。方法使用IL-6处理MLE12细胞构建炎性肺上皮细胞模型,采用随机数表法将细胞分成5组:空白对照组(C组)培养基中无干预培养96 h;IL-6组(I组)向培养基中加入50 ng/mL IL-6;IL-6+右美托咪定组(I+D组)向培养基中加入50 ng/mL IL-6,24 h后向培养基中加入10μM DEX;IL-6+HY-121488组(I+H组)向培养基中加入gp130激动剂HY-12148850μM后再向培养基中加入50 ng/mL IL-6;IL-6+HY-121488+DEX组(I+H+D组)向培养基中加入50μM HY-121488后向培养基中加入50 ng/mL IL-6,24 h后向培养基中加入10μM DEX。72 h后收集各组细胞用于实验检测。采用PCR检测细胞IL-6、JAK2、STAT3的mRNA表达水平。结果与C组比较,I组IL-6、JAK2、STAT3 mRNA表达水平均升高(P<0.05);与I组比较,I+D组IL-6、JAK2、STAT3 mRNA表达水平均降低(P<0.05);与I+D组比较,I+H+D组IL-6、JAK2、STAT3 mRNA表达水平均升高(P<0.05);与I组比较,I+H组IL-6、JAK2、STAT3 mRNA表达水平均升高(P<0.05);与I+H组比较,I+H+D组IL-6、JAK2、STAT3 mRNA表达水平均降低(P<0.05)。结论右美托咪定通过调控gp130介导的JAK2/STAT3信号通路减轻炎症反应,对肺组织发挥保护作用。

Objective To investigate the effect and mechanism of dexmedetomidine(Dex)on JAK2/STAT3 signaling pathway in interleukin-6(IL-6)-induced inflammatory alveolar epithelial cell model.Methods Inflammatory alveolar epithelial cell model was constructed by treating MLE12 cells with IL-6,and the cells were divided into five groups by random number method:the blank control group(group C)was cultured without intervention in culture medium for 96 h;the IL-6 group(group I)was added with 50 ng/ml IL-6 into the culture medium;and the IL-6+dexmedetomidine group(group I+D)was added with 50 ng/ml IL-6 into the culture medium,and after 24 h,10μM DEX was added to the culture medium;IL-6+HY-121488 group(Group I+H)added 50 ng/ml IL-6 to the culture medium after adding 50μM HY-121488;IL-6+HY-121488+DEX group(Group I+H+D)after adding 50μM HY-121488 to the medium,50 ng/ml IL-6 was added to the medium and 10μM DEX to the medium 24 h later.Cells in each group were collected for experimental detection after 72 h.The mRNA expression levels of IL-6,JAK2 and STAT3 were detected by PCR.Results Compared with group C,the mRNA expression levels of IL-6,JAK2,and STAT3 mRNA were increased in group I(P<0.05);compared with group I,the mRNA expression levels of IL-6,JAK2,and STAT3 mRNA were decreased in group I+D(P<0.05);and compared with group I+D,the mRNA expression levels of IL-6,JAK2,and STAT3 mRNA were increased in group I+H+D(P<0.05);compared with group I,IL-6,JAK2,and STAT3 mRNA expression levels were all increased in group I+H(P<0.05);compared with group I+H,IL-6,JAK2,and STAT3 mRNA expression levels were all decreased in group I+H+D(P<0.05).Conclusion Dexmedetomidine attenuates the inflammatory response and exerts a protective effect on lung tissues by modulating the gp130-mediated JAK2/STAT3 signaling pathway.