肠道微生物群与口腔癌发生风险的两样本孟德尔随机化研究

Correlation between gut microbiota and oral cancer risk:insights from a two-sample Mendelian randomization analysis

目的使用孟德尔随机化(MR)分析探讨肠道微生物群与口腔癌风险的相关性。方法从OPEN GWAS数据库与MiBioGen联盟分别下载口腔癌与肠道微生物群的汇总数据,将IVW方法作为主要的统计学方式,其他4种MR-Egger,Weighted median,Weighted mode和simple mode作为补充方式,采用双样本MR分析探索肠道微生物菌群与口腔癌风险间的因果关系。此外,敏感性分析用于检测异质性和多效性。结果采用IVW作为主要的统计方法。结果发现,Betaproteobacteria(OR=1.001105,P=0.01576412,95%CI:1.0002078~1.002002)、Alcaligenaceae(OR=1.001249,P=0.01154315,95%CI:1.0002797~1.002219)、Pasteurellales(Pasteurellaceae)(OR=1.000510,P=0.03808085,95%CI:1.0000280~1.000993)、Eubacterium fissicatena group(OR=1.000488,P=0.03155466,95%CI:1.0000431~1.000932)和Desulfovibrio(OR=1.000714,P=0.04466106,95%CI:1.0000170~1.001412)对口腔癌提示有促进作用,而Butyrivibrio(OR=0.9996003,P=0.02473086,95%CI:0.9992516~0.9999492)和Clostridium sensu stricto 1(OR=0.9986777,P=0.001871154,95%CI:0.9978452~0.9995109)对口腔癌提示具有保护作用。结论MR分析评估肠道菌群与口腔癌之间的因果关系,发现5个菌属(Pasteurellaceae与Pasteurellales同属一种)促进口腔癌的发展,而2个菌属对口腔癌具有保护作用。

Objective To explore the potential causal association between gut microbiota and oral cancer risk using Mendelian randomization(MR)analysis.Methods Summarized data on oral cancer and gut microbiota were downloaded from the OPEN GWAS database and the MiBioGen consortium,respectively.A two-sample MR analysis was performed,employing the inverse-variance weighted(IVW)method as the primary statistical approach,complemented by MR-Egger,weighted median,weighted mode,and simple mode methods.This analysis aimed to investigate the causal relationship between gut microflora and oral cancer risk.In addition,sensitivity analyses were used to detect heterogeneity and pleiotropy.Results Using the IVW method as the primary statistical approach,the results revealed that Betaproteobacteria(OR=1.001105,P=0.01576412,95%CI:1.0002078~1.002002)、Alcaligenaceae(OR=1.001249,P=0.01154315,95%CI:1.0002797~1.002219)、Pasteurellales(Pasteurellaceae)(OR=1.000510,P=0.03808085,95%CI:1.0000280~1.000993)、Eubacterium fissicatena group(OR=1.000488,P=0.03155466,95%CI:1.0000431~1.000932)and Desulfovibrio(OR=1.000714,P=0.04466106,95%CI:1.0000170~1.001412)were associated with an increased risk of oral cancer,suggesting a potential promoting effect.In contrast,Butyrivibrio(OR=0.9996003,P=0.02473086,95%CI:0.9992516~0.9999492)and Clostridium sensu stricto 1(OR=0.9986777,P=0.001871154,95%CI:0.9978452~0.9995109)had an inhibitory effect on oral cancer development.Conclusion The MR analysis evaluating the causal relationship between gut microbiota and oral cancer reveals that five bacterial genera(including Pasteurellaceae and Pasteurellales,which belong to the same genus)are associated with an increased risk of oral cancer,while two genera exhibit a protective effect.