苯并[a]芘厌氧降解菌群的富集及其条件优化

Enrichment of benzopyrene anaerobic degrading bacteria and optimization of its conditions

从污泥-秸秆联合厌氧污泥体系中富集出以BaP为唯一碳源的菌群——YQ-BaP菌群,通过单因素实验研究了不同环境因素对菌群降解能力的影响,采用GC-MS分析苯并[a]芘降解的中间代谢产物。结果表明:该菌群优势菌属为Pseudomonas和Methyloversatilis。该菌群在培养14 d后对30 mg/L BaP的降解率稳定在38.69%±6.24%,对菲和芘的降解效果更佳。YQ-BaP菌群在pH=7和35~40℃时表现出较强的降解能力;当BaP初始浓度为20 mg/L时,对BaP降解率达到46.89%。GC-MS分析表明,BaP在降解过程中产生菲、蒽和萘等羟化和甲基化代谢产物。研究显示,该菌群具有较强的BaP降解能力,可为多环芳烃污染环境的生物修复提供理论基础和技术支撑。

The YQ-BaP microbial community with Benzo[a]pyrene(BaP)as the sole carbon source was enriched from the sludge straw combined anaerobic sludge system.The effects of different environmental factors on the biodegradability of bacteria were studied by single factor experiment.The intermediate metabolites degraded by benzo[a]pyrene were analyzed by gas chromatography-mass spectrometry(GC-MS).The results of macrogene sequencing showed that the dominant genera of this bacterium were Pseudomonas and Methyloversatilis.After 14 days of culture,the degradation rate of 30 mg/L BaP was stable at 38.69%±6.24%,and the degradation effect of phenanthrene and pyrene was better.YQ-BaP showed strong degradation ability at pH=7 and 35~40℃.When the concentration of BaP was 20 mg/L,the degradation rate of BaP reached 46.89%.GC-MS analysis showed that phenanthrene,anthracene,naphthalene and other hydroxylation and methylation metabolites were produced during BaP degradation.The bacterial community has a strong ability to degrade BaP,which provides a theoretical basis for the remediation technology of PAHs pollution.