人畜互动对新生犊牛生长性能及血清免疫、应激和生化指标的影响

Effects of Human-Animal Interaction on Growth Performance and Serum Immune,Stress and Biochemical Indices of Newborn Calves

本试验旨在研究人畜互动对新生犊牛生长性能及血清免疫、应激和生化指标的影响。选取32头初生重相近的健康荷斯坦母犊牛,随机分为4组,每组8个重复,每个重复1头牛。按摩组(HM组)由试验人员用手持续按摩犊牛颈下部(60次/min),刷拭组(HB组)由试验人员使用小型体毛刷持续刷拭犊牛颈下部(60次/min),简单互动组(HI组)由试验人员靠近犊牛站立间歇触碰其背部10次(5次/min),对照组(CON组)犊牛不进行人畜互动。试验期为14 d,每天2次(10:00和16:00)人畜互动,每次2 min。试验期间测定犊牛生长性能,在试验第1天和第14天收集血样,检测血清免疫、应激和生化指标。结果表明:1)各组之间犊牛生长性能无显著差异(P>0.05)。2)刷拭组血清免疫球蛋白A(IgA)含量显著高于对照组(P<0.05)。3)相比1日龄,经过14 d人畜互动后,刷拭组、按摩组和对照组犊牛血清IgA含量增加幅度显著高于简单互动组(P<0.05),刷拭组和按摩组犊牛血清白细胞介素-10含量增加幅度显著高于简单互动组(P<0.05),刷拭组、按摩组和简单互动组犊牛血清皮质醇含量下降幅度显著高于对照组(P<0.05)。综上所述,体毛刷刷拭可以增强新生犊牛免疫力,降低新生犊牛的应激水平,提示体毛刷刷拭可能是一种改善新生犊牛健康和福利的有效互动方式。

The purpose of this experiment was to study the effects of human-animal interaction on growth performance and serum immune,stress and biochemical indices of newborn calves.Thirty-two healthy newborn Holstein female calves with similar birth weight were randomly divided into 4 groups with 8 replicates per group and 1 calf per replicate.In the massage group(HM group),calves were continuously massaged by the experimenter on the lower part of their necks(60 times/min);in the brushing group(HB group),calves were continuously brushed on the lower part of their necks using a small body brush by the experimenter(60 times/min);in the simple interaction group(HI group),calves were approached by the experimenter for 10 intermittent contacts on the back while they were standing(5 times/min);in the control group(CON group),calves did not receive any human-animal interaction.The testing period lasted for 14 days,with two times of human-animal interaction per day(10:00 and 16:00)for 2 minutes in each time.During the test,the growth performance of calves was measured,and collected the blood samples on day 1 and day 14 to measure the serum immune,stress and biochemical indices.The results showed as follows:1)there was no significant difference in the growth performance of calves among all groups(P>0.05).2)The serum immunoglobulin A(IgA)content of the brushing group was significantly higher than that of the control group(P<0.05).3)Compared to 1 day of age,after 14 days of human-animal interaction,the increase extent of serum IgA content of brushing group,massage group and control group was significantly higher than that of the simple interaction group(P<0.05),the increase extent of serum interleukin-10 content of brushing group and massage group was significantly higher than that of the simple interaction group(P<0.05),and the decrease extent of serum cortisol content of brushing group,massage group and simple interaction group was significantly higher than that of the control group(P<0.05).In conclusion,the body brush swabbing can enhance the immunity and reduce the stress levels of newborn calves,suggesting that body brush swabbing may represent an effective interaction to improve newborn calves’health and welfare.