B7-H3在结直肠癌中的表达特征分析和临床意义

Expression characteristics and clinical significance of B7-H3 in colorectal cancer

目的探讨B7同源物3(B7-H3)在结直肠癌(CRC)组织中的表达特征及临床意义,探究其与肿瘤糖酵解过程以及与肿瘤微环境中免疫细胞浸润的相关性。方法从肿瘤基因组图谱数据库(TCGA)下载CRC转录组数据和临床病理数据,分析B7-H3的表达情况和临床意义。基于TCGA数据分析CRC内糖酵解相关基因表达与B7-H3表达的相关性。使用通过估计RNA转录物相对子集的细胞类型鉴定(CIBERSORT)算法探究B7-H3表达与22种免疫细胞浸润的关系。收集51例CRC患者的手术标本及癌旁正常组织标本,应用免疫组织化学染色方法对数据库分析结果进行验证。结果B7-H3在CRC组织中的表达上调,且与肿瘤浸润深度、TNM分期晚期和不良预后密切相关。此外,B7-H3表达与CRC内糖酵解相关基因上调密切相关。免疫细胞浸润分析显示共有16种免疫细胞的浸润水平与B7-H3表达密切相关,进一步发现B7-H3表达与CD8+T细胞浸润在转录水平上呈负相关,但两者在蛋白水平上不相关。结论B7-H3在CRC组织中高表达,与CRC疾病进展和患者的不良预后密切相关。此外,B7-H3与糖酵解相关基因以及免疫细胞浸润之间的相关性,提示B7-H3在调控肿瘤糖酵解和肿瘤免疫中起到关键作用。

Objective To elucidate the expression characteristics and clinical significance of B7 homolog 3(B7-H3)in colorectal cancer(CRC)and to explore its associations with tumor glycolysis and immune cell infiltration in the tumor microenvironment.Methods The transcriptomic and clinicopathological data of CRC were obtained from the TCGA database and analyzed to determine the expression and clinical relevance of B7-H3.Correlations between glycolysis-related genes and B7-H3 expression were assessed based on TCGA data.The associations between B7-H3 expression and the infiltration of 22 types of immune cells were analyzed using the CIBERSORT algorithm.Immunohistochemical staining was used to verify the results of database analysis in surgical specimens and adjacent normal tissue specimens of 51 CRC patients.Results B7-H3 was significantly upregulated in CRC tissues and demonstrated strong correlations with tumor invasion depth,advanced TNM stage,and poor prognosis.Additionally,B7-H3 expression was closely associated with the upregulation of glycolysis-related genes in CRC.Immune cell infiltration analysis revealed that a total of 16 types of immune cells were significantly correlated with B7-H3 expression.Furthermore,an inverse relationship between B7-H3 expression and CD8+T cell infiltration was identified at the transcriptional level but not at the protein level.Conclusion B7-H3 is highly expressed in CRC tissues and is significantly correlated with disease progression and poor prognosis.Furthermore,the association of B7-H3 expression with glycolysis-related genes and immune cell infiltration suggests a pivotal role of B7-H3 in the regulation of tumor glycolysis and cancer immunity.