Box-Behnken响应面法优化山蜡梅精油的提取工艺及其化学成分研究

Study on optimization of Box-Behnken response surface method extraction and chemical composition analysis of essential oil from Chimonanthus nitens Oliv.

目的:利用响应面法优化山蜡梅叶精油的提取工艺,并采用GC-MS技术分析山蜡梅精油化学成分。方法:通过设计Box-Behnken响应面模型,考察粉碎粒度、浸泡时间、浸提时间和液料比对山蜡梅精油提取率的影响。再利用响应面模型对实验结果进行拟合,确定最优组合条件,采用GC-MS分析山蜡梅精油的化学成分。结果:最佳提取工艺为:粉碎粒度24目、浸泡时间1.4 h、提取时间3.1 h、液料比31∶1 mL/g,在此条件下的精油得率可达7.60%。采用优化后同时蒸馏法提取所得山蜡梅精油中,共检测出56种化合物。其中,醇类12种,相对含量为47.98%;烯烃类化合物33种,相对含量为25.11%;酯类、烷类及醛类化合物相对含量为7.82%。结论:同时蒸馏法较传统的水蒸气蒸馏法提取更加彻底。优化后的山蜡梅精油提取工艺稳定、可靠,此研究可为山蜡梅精油产品的进一步开发提供一定的理论依据。

Objective:To optimize the extraction conditions of essential oil from Chimonanthus nitens Oliv.by using Box-Behnken response surface methodology,and to study its chemical components by GC-MS.Methods:By designing BoxBehnken response surface model,the effects of crushing particle size,soaking time,extraction time and liquid material ratio on the extraction rate of Chimonanthus nitens Oliv.essential oil were investigated.Then the response surface model is used to fit the experimental results to determine the final optimal combination conditions,The chemical constituents of essential oil from Chimonanthus nitens Oliv.were analyzed by GC-MS.Results:The optimal technological conditions for extraction of Chimonanthus nitens Oliv.was as follows:The yield of essential oil can reach 7.60% under the conditions of crushing particle size of 24 mesh,soaking time of 1.4 h,extraction time of 3.1 h and liquid-material ratio of 31∶1 mL/g;A total of 56 compounds were extracted by optimized simultaneous distillation-extraction,including 12 alcohols,accounting for 47.98%,33 olefins,with The relative content of 25.11%,and other compounds,including esters,alkys and aldehydes,accounted for 7.82%.Conclusion:the optimized extraction process of Chimonanthus nitens Oliv.essential oil is stable and reliable.This study can provide a theoretical basis for the further development of Chimonanthus praecox essential oil products.