GNL3蛋白在胃癌中的表达及其在细胞增殖、侵袭以及迁移中的作用

Expression of GNL3 protein in gastric cancer and its effect on cell proliferation,invasion and migration

目的探讨鸟嘌呤核苷酸结合蛋白样3(guanine nucleotide binding protein-like 3,GNL3)蛋白在胃癌中的表达,探明GNL3蛋白在胃癌增殖、迁移及侵袭等生物学功能中的作用。方法数据库分析GNL3 mRNA在胃癌组织中的表达情况;采用免疫组化SP法检测51例胃癌组织和对应癌旁正常组织中GNL3蛋白表达;χ^(2)检验分析GNL3蛋白表达与胃癌临床病理特征之间的关系;通过转染小发夹RNA(small hairpin RNA,sh-RNA)沉默胃癌细胞中GNL3的表达,并应用Western blot法验证沉默效率。采用CCK-8、平板克隆和EdU实验检测沉默GNL3对胃癌细胞增殖能力的影响。采用细胞划痕和Transwell实验检测沉默GNL3对胃癌细胞侵袭和迁移能力的影响。结果SangerBox和UALCAN数据库检索结果显示,GNL3 mRNA在胃癌组织中的表达显著增加(P均<0.01)。免疫组化结果显示,GNL3蛋白在胃癌组织中的阳性率(96.1%)和高表达率(78.4%)显著高于癌旁组织(74.5%、51.0%,P<0.01),并且GNL3高表达与胃癌淋巴结转移(χ^(2)=4.933,P=0.026)具有相关性。CCK-8、平板克隆以及EdU实验结果显示,沉默GNL3可抑制胃癌细胞SGC-7901的增殖能力。细胞划痕和Transwell实验结果显示,沉默GNL3可抑制胃癌细胞SGC-7901的迁移和侵袭能力。结论GNL3蛋白在胃癌组织中高表达,且与胃癌细胞增殖、迁移及侵袭能力密切相关。

Purpose To investigate the expression status of GNL3 in gastric cancer,and to explore the role of GNL3 in tumor proliferation,invasion and migration.Methods The expression of GNL3 mRNA in gastric cancer tissues was analyzed by searching database.The expression of GNL3 protein in 51 gastric cancer tissues and 51 adjacent non-tumor tissues was detected by immunohistochemistry(IHC)SP method.The correlation between GNL3 protein expression and gastric cancer clinical pathological features was analyzed byχ^(2)test.The expression of GNL3 in gastric cancer cells was silenced by transfection of sh-RNA,and the silencing efficiency was verified by Western blot.The effect of silencing GNL3 on the proliferation of gastric cancer cells was examined by CCK-8,colony formation and EdU staining.In addition,wound-healing assay and Transwell assay were performed to detect the effect of GNL3 silencing on cell invasion and migration.Results SangerBox and UALCAN database retrieval showed that the expression of GNL3 mRNA was significantly increased in gastric cancer tissues(P<0.01).IHC staining showed that the positive expression rate of GNL3 protein in gastric cancer tissues was 96.1%,and the high expression rate was 78.4%,which was significantly higher than that in adjacent non-tumor tissues(74.5%,51.0%,P<0.01).Moreover,the high expression of GNL3 was significantly correlated with lymph node metastasis in gastric cancer patients(χ^(2)=4.933,P=0.026).CCK-8,colony formation and EdU staining showed that GNL3 silencing inhibited the proliferation of gastric cancer cell SGC-7901.The wound-healing and Transwell assay showed that GNL3 silencing inhibited the migration and invasion of gastric cancer cell.Conclusion The GNL3 protein is highly expressed in gastric cancer tissues,and closely related to the proliferation,migration and invasion of gastric cancer cells.