洋葱转录组测序及黄酮类化合物合成相关基因的分析

Transcriptome Sequencing and Analysis of Flavonoids Synthesis Related Genes in Onion

洋葱是世界范围内种植的重要蔬菜作物,其鳞茎颜色是洋葱选育和食用的重要性状。本研究对紫皮洋葱和黄皮洋葱进行高通量转录组测序,挖掘黄酮类化合物合成的基因,探讨不同皮色形成的分子基础。本研究共获得5 809个差异表达基因,包括2 991个上调表达基因,2 818个下调表达基因。差异基因GO功能富集分析显示,差异基因主要参与细胞组分、生物过程和分子功能3大类。KEGG显著富集分析显示,差异表达基因主要富集在3条代谢途径上。黄酮类化合物合成相关的通路有类黄酮合成途径、花青素合成途径、异黄酮合成途径、黄酮和黄酮醇合成途径,并筛选出了F3’H、FLS、CHI和DFR等黄酮类合成相关的结构基因。本研究为进一步探讨洋葱皮色相关的代谢合成途径关键酶的功能及其调控机制提供了基础。

Onion(Allium cepa L.)is an important vegetable crop grown worldwide and its bulb color is an important trait for onion selection and consumption.In this study,high-throughput transcriptome sequencing was performed on red onions and yellow onions,to explore related genes of flavonoids synthesis and their molecular basis of different colors.The results showed that a total of 5809 differentially expressed genes were obtained,including 2991 up-regulated genes and 2818 down-regulated genes.The GO function enrichment analysis of differential gene showed that differential genes were mainly involved in three categories:cellular component,biological process and molecular function.KEGG significant enrichment analysis showed that differentially expressed genes were mainly enriched in three metabolic pathways.The main enrichment pathways were related to flavonoids synthesis including flavonoid biosynthesis,anthocyanin biosynthesis,isoflavonoid biosynthesis,flavone and flavonol biosynthesis,and the structural genes related to flavonoid synthesis such as F3'H,FLS,CHl,DFR were screened.This study provides a basis for further exploring the functions of key enzymes of the metabolic synthesis pathways related to onion color and their regulatory mechanisms.