吉首市八月湖桃花水母的鉴定

Identification Analysis on Craspedacusta sp.from Bayue Lake in Jishou City

为了加强对桃花水母的保护及可持续利用,采用形态学鉴定和分子生物学鉴定相结合的方法,对吉首市八月湖的桃花水母进行形态特征分析,并基于18S rDNA和ITS基因序列计算不同桃花水母的种间遗传距离,构建系统发育树.结果表明,吉首市八月湖桃花水母伞径平均12.4 mm,触手3~4级、主辐触手明显长于其他触手,生殖腺囊状、呈褐黄色,平衡囊管形、106~128个,刺丝囊疣呈环形排列;吉首市八月湖桃花水母与美国索式桃花水母(GenBank序列号为MG979336.1)的18S rDNA基因序列相似度最高,为99.88%,与索氏桃花水母亲缘关系较近.形态数据及分子数据均支持八月湖桃花水母为索氏桃花水母.

In order to strengthen the conservation and sustainable utilization of Craspedacusta,we used the methods of morphological identification and molecular biological identification to identify and analyze the Craspedacusta sp.from Bayue Lake in Jishou City of Xiangxi.The results were as follows:Diameter of umbrella was 12.4 mm.Tentacle 3~4,and the main tentacle was significantly longer than other tentacles.The gonads were cystic,and brown yellow in color.Statocysts were tubular,106~128 in number.Nematocyst warts arranged in a ring and each of ring had interval.The similarity of 18S rDNA gene sequence between Craspedacusta sp.from Bayue Lake and Craspedacusta sowerbyi of the United States(GenBank serial number MG979336.1)was the highest,which was 99.88%.The relationship between the Craspedacusta sp.from Bayue Lake in Xiangxi and Craspedacusta sowerbyi was close.Both morphological and molecular data support that the Craspedacusta sp.from Bayue Lake in the Xiangxi was Craspedacusta sowerbyi.