艳山姜挥发油对L-NAME诱导高血压大鼠心脏损伤的保护作用

Protective effect of essential oils from Fructus Alpiniae zerumbet on cardiac injury in L-NAME-induced hypertensive rats

目的:研究艳山姜挥发油(essential oils from Fructus Alpiniae zerumbet,EOFAZ)对Nω-硝基-L-精氨酸甲酯盐酸盐(Nω-nitro-L-arginine methyl ester hydrochloride,L-NAME)诱导高血压大鼠心脏损伤的保护作用。方法:L-NAME溶液口服灌胃4周建立高血压大鼠模型,模型成功的大鼠分别给予EOFAZ和卡托普利治疗4周。每周采用无创血压测量系统监测大鼠动脉血压;小动物B超成像系统检测大鼠心脏功能;HE染色观察大鼠心脏组织病理变化;比色法检测大鼠心脏中MDA、SOD、NO水平;免疫组化法检测大鼠心脏中eNOS、iNOS表达;Western blot法检测大鼠心脏中炎症相关蛋白TNF-α、IL-1β,氧化应激相关蛋白Nrf2、HO-1、Prdx-1,心脏肥厚相关蛋白ANP、BNP,以及eNOS、iNOS蛋白表达。结果:EOFAZ显著降低高血压大鼠血压,改善大鼠心脏结构异常和功能障碍,降低MDA和NO水平,增加SOD活性,下调ANP、BNP、TNF-α、IL-1β、iNOS表达,以及上调Nrf2、HO-1、Prdx-1、eNOS表达(P<0.05,P<0.01)。结论:EOFAZ对L-NAME诱导的高血压大鼠心脏损伤具有明显保护作用,其作用与抑制炎症、改善氧化应激、提高NO生物利用度有关。

OBJECTIVE To explore the protective effect of essential oils from Fructus Alpiniae zerumbet(EOFAZ)on Nω-nitro-L-arginine methyl ester hydrochloride(L-NAME)-induced cardiac injury in hypertensive rats.METHODS Cardiac damage of rat was induced by a continuous intragastric dose of L-NAME for 4 weeks and EOFAZ/captopril for 4 weeks.Arterial blood pressure was monitored weekly using a noninvasive blood pressure measurement system.Animal ultrasonic imaging system was employed for observing cardiac function.Pathological changes of cardiac tissue were observed by HE stain.The cardiac levels of malondialdehyde(MDA),SOD and NO were measured by colorimetry.The expressions of eNOS and iNOS in rat heart were detected by immunohistochemical assay.Western blot was utilized for detecting oxidative stress-related proteins Nrf2,HO-1 and Prdx-1,inflammation-related proteins TNF-αand IL-1β,cardiac hypertrophy-associated proteins ANP and BNP,as well as eNOS and iNOS protein expression in rat hearts.RESULTS EOFAZ significantly reduced blood pressure,ameliorated cardiac structural abnormalities and dysfunction,decreased MDA and NO levels,increased SOD activity,down-regulated the expression of ANP,BNP,TNF-α,IL-1βand iNOS,as well as up-regulated the expression of Nrf2,HO-1,Prdx-1 and eNOS in hyperten⁃sive rats(P<0.05,P<0.01).CONCLUSION EOFAZ could ameliorate cardiac injury in L-NAME-induced hypertensive rats,the mechanism maybe involve in inhibiting inflammation and regulating oxidative stress,as well as the promotion of increased NO bioavailability.