玫瑰高温水萃溶液中多酚化合物的研究及应用

Study and Application of Polyphenol Compounds in Rose Wastewater

通过有机溶剂萃取的方法制备大马士革玫瑰(Rosa Damascena Mill.)高温水萃溶液的多酚中间体,中间体中多酚含量为高温水萃溶液的3.7倍,利用UPLC-QTRAP鉴定并定量分析12种多酚化合物(没食子酸、花青素、金丝桃苷、槲皮苷等),其中花青素含量最为丰富,占中间体总量的20%。抗氧化活性体外测试结果表明,玫瑰多酚中间体对ABTS+、DPPH·自由基的清除能力与VC相当。以多酚中间体为原料制备多酚片剂和饮料,均具有不同程度的抗氧化能力,且多酚片剂对酪氨酸酶的活性具有一定抑制作用。试验结果可为进一步开发玫瑰精油生产过程中产生的副产物高温水萃溶液作为功能性食品配料提供依据。

The polyphenol intermediate of Rosa Damascena Mill. wastewater was prepared by organic solvent extraction. The content of polyphenol in the intermediate was 3.7 times of that in the wastewater. Twelve polyphenol compounds(anthocyanin,gallic acid, hypericin, quercetin, etc) were identified by UPLC-QTRAP. Among them, anthocyanins made up 20% of the total intermediates and were the most common compound. In vitro experiments showed that the ability of polyphenol intermediates to eluate ABTS and DPPH intermediates was as strong as that of VC, and it had good antioxidant activity. Polyphenol tablets and beverages prepared from polyphenol intermediates showed antioxidant activity of different abilities, and polyphenol tablets also had the activity of inhibiting tyrosinase. The results can provide a basis for further developing the wastewater produced in the production of rose essential oil as a functional food ingredient.