魔芋葡甘露聚糖凝胶过程脱小分子的研究

Study on the Removal of Small Molecules in Konjac Glucomannan Gel Process

探讨魔芋葡甘露聚糖(KGM)水溶胶在NaOH作用下形成不可逆凝胶过程脱出的小分子物质。试剂KGM及魔芋地下块茎干粉制成水溶胶后加入NaOH溶液放置20 h,然后蒸馏。硫酸吸收蒸馏液,用离子色谱法检测胺类,参照HJ534-2009定量检测NH_(3)。蒸馏后的残留液加磷酸调整为强酸性再次蒸馏收集挥发性酸,用气相色谱法检测酸性物质,参照NY 82.16-1988定量检测总挥发性酸。结果表明:试剂KGM、魔芋地下块茎干粉在NaOH作用下都能脱NH_(3)和甲酸根,脱NH_(3)量分别为0.009 9 mol/100 g和0.005 3 mol/100 g,脱总挥发性酸分别为0.034 3 mol/100 g和0.014 9 mol/100 g。KGM在碱性环境下脱出NH_(3)及甲酸根形成不可逆凝胶。

Study the small molecules that are removed from Konjac Glucomannan(KGM) hydrosol by irreversible gel formation process under the action of NaOH. Reagent konjac glucomannan(KGM) and Konjac dry underground tuber powder are prepared into hydrosol and placed in NaOH solution for 20 h, then distilled. The distilled solution is absorbed by sulfuric acid. Amines are detected by ion chromatography, and NH_(3) is quantitatively detected according to HJ 534-2009. The residual liquid after distillation is adjusted to strong acid with phosphoric acid, and the volatile acid is collected by distillation again.The acidic substance is detected by gas chromatography, and the total volatile acid is quantitatively detected according to NY 82.16-1988. The results show that, reagent KGM and Konjac dry underground tuber powder can remove NH_(3) and formate under the action of NaOH, the amount of NH_(3) removed is 0.009 9 mol/100 g and 0.005 3 mol/100 g, respectively, and the total volatile acid removed is 0.034 3 mol/100 g and 0.014 9 mol/100 g, respectively. KGM can release NH_(3) and formate to form irreversible gel in alkaline environment.