我国罕见G9P[8]-A2基因型重配株轮状病毒全基因组分子特征分析

Genome-wide molecular characterization analysis of rare G9P[8]-A2 genotype rotavirus in China

目的 了解我国1株G9P[8]基因型A组轮状病毒(RVA)JL18221140的全基因组分子特征。方法 对我国吉林省1例G9P[8]基因型RVA(JL18221140)原始粪便标本采用反转录-聚合酶链式反应(RT-PCR)一步法扩增11个基因节段,并通过DNAStar, MEGA 11.0等生物信息分析软件进行同源性及进化分析。结果 JL18221140的全基因组11个节段的基因型为G9-P[8]-I1-R1-C1-M1-A2-N1-T1-E1-H1,NSP1基因型呈现DS-1样特征。系统发育分析显示VP7和VP4分别聚集在谱系G9-Ⅵ和P[8]-Ⅲ中。结论 JL18221140为G9P[8]基因型中罕见的G9P[8]-A2重配株,该基因型可能是由中国流行的G9P[8]-A1和G2P[4]-A2毒株共感染过程中基因组间重配产生的。表明对RVA持续监测并对全基因组测序的重要性,有利于了解我国RVA毒株的遗传变异情况,为今后病毒的防控和疫苗的研发提供更有效的基础数据。

Objective To investigate the genome-wide molecular characteristics of group A rotavirus(RVA)JL18221140,a G9P[8]genotype in China.Methods A raw fecal specimen of G9P[8]genotype RVA(JL18221140)in Jilin province,China,was amplified by reverse transcription-polymerase chain reaction(RT-PCR)one-step method to amplify 11 gene segments,and homology and evolution analysis were carried out by DNAStar,MEGA 11.0 and other bioinformatics analysis software.Results The genotypes of 11 segments of the whole genome of JL18221140 were G9-P[8]-I1-R1-C1-M1-A2-N1-T1-E1-H1,and the NSP1 genotype showed DS-1-like characteristics.Phylogenetic analysis showed that VP7 and VP4 were clustered in lineages G9-VI and P[8]-Ⅲ,respectively.Conclusion JL18221140 is a rare G9P[8]-A2 reassortant strain among G9P[8]genotypes,which may have arisen from inter-genomic reassortment during co-infection with the G9P[8]-A1 and G2P[4]-A2 strains prevalent in China.This shows the importance of continuous monitoring of RVA and whole genome sequencing,which is conducive to understanding the genetic variation of RVAstrains in China,and provides more effective basic data for future virus prevention and control and vaccine research and development.