大承气汤对脓毒症胃肠功能障碍大鼠胃肠功能及炎症因子的影响

Effects of the Dachengqi decoction on gastrointestinal function and inflammatory factors in rats with sepsis gastrointestinal dysfunction

目的:研究大承气汤对脓毒症胃肠功能障碍模型大鼠胃肠运动功能及炎症因子的影响。方法:将50只雄性Wistar大鼠随机分为假手术组、模型组、大承气汤高(10 g/kg)剂量组、大承气汤中(5 g/kg)剂量组、大承气汤低(2.5 g/kg)剂量组,每组10只。除假手术组,其余采用经典盲肠结扎穿刺法制备脓毒症胃肠功能障碍模型大鼠,造模后,大承气汤各组分别予相应剂量药液,假手术组与模型组予等量生理盐水,连续灌胃7 d,每天1次。给药结束后,采用半固体糊法测定各组大鼠胃残留率及肠推进率,利用酶联免疫吸附法检测各组大鼠血清胃肠激素胃动素(Motilin,MTL)、胃泌素(Gastrin,GAS)及炎症因子肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白细胞介素(Interleukin,IL)-1β水平。结果:与假手术组比较,模型组大鼠胃排空率、肠推进率、MTL、GAS水平显著降低,而TNF-α、IL-1β水平显著升高,差异有显著统计学意义(P<0.01);与模型组比较,大承气汤各组大鼠胃排空率、肠推进率均不同程度升高,差异有显著统计学意义(P<0.01),大承气汤高剂量组、大承气汤中剂量组MTL、GAS水平明显升高,差异有显著统计学意义(P<0.01),大承气汤各组大鼠TNF-α水平均显著降低,差异有显著统计学意义(P<0.01),而大承气汤高、中剂量组大鼠IL-1β水平显著降低,差异有显著统计学意义(P<0.01)。结论:大承气汤可有效促进脓毒症胃肠功能障碍大鼠胃肠运动,提高胃肠激素分泌水平,同时可抑制炎症因子释放,减少脓毒症炎症反应。

Objective:To study the effects of the Dachengqi decoction(大承气汤)on gastrointestinal function and inflammatory factors in rats with septic gastrointestinal dysfunction.Methods:Fifty male Wistar rats were randomly divided into the sham-operated group,model group,the Dachengqi decoction high(10 g/kg),medium(5 g/kg)and low(2.5 g/kg)dose groups,with ten rats in each group.Except for sham-operated group,the rest were given cecal ligation and perforation to estabish septic gastrointestinal dysfunction models.The Dachenqi decoction groups were given corresponding dose of the Dachenqi decoction,the sham-operated group and the model group were given the equal amounts of physiological saline,were continuously gavaged once a day,for 7 days.After administration,the gastric residual rate and intestinal advancement rate of rats were measured by semi-solid paste method,and the serum levels of gastrointestinal hormones motilin,gastrin and inflammatory factors tumor necrosis factor-αand interleukin-1βwere detected by enzyme-linked immunosorbent assay.Results:Compared with the sham-operated group,gastric emptying rate,intestinal propulsion rate,motilin and gastrin levels in the model group were significantly decreased,while tumor necrosis factor-αand interleukin-1βlevels were significantly increased,with significant statistical differences(P<0.01).Compared with the model group,the gastric emptying rate and intestinal propulsion rate of rats in each dose groups of the Dachengqi decoction were increased to varying degrees,with significant statistical differences(P<0.01);the levels of motilin and gastrin in the high-dose and medium-dose groups of the Dachengqi decoction were significantly increased,with significant statistical differences(P<0.01).The level of tumor necrosis factor-αin each dose groups of the Dachengqi decoction were significantly decreased,and the differences were statistically significant(P<0.01),while the level of interleukin-1βin the high-dose and medium-dose groups of Dachengqi decoction were significantly decreased,and the differences were statistically significant(P<0.01).Conclusion:Dachengqi decoction could effectively promote gastrointestinal motility in rats with septic gastrointestinal dysfunction,increase the secretion level of gastrointestinal hormones,inhibit the release of inflammatory factors,and reduce the inflammatory reaction of sepsis.