槲皮万寿菊素对氧化应激下肉鸡抗氧化能力和免疫功能的影响

Effects of quercetagetin on antioxidant capacityand immune function of broilers under oxidative stress

试验旨在探究饲粮中添加槲皮万寿菊素(QG)对氧化应激下肉鸡抗氧化能力和免疫功能的影响。选取240只1日龄科宝肉鸡,随机分为对照组、敌草快氧化应激组和QG组,每组8个重复,每个重复10只肉鸡。对照组和敌草快氧化应激组饲喂基础饲粮,QG组饲喂在基础饲粮中添加100 mg/kg QG的试验饲粮。试验期23 d。试验第20 d,从每个重复选取1只接近该笼平均体重的肉鸡,对照组腹腔注射1 mL生理盐水,敌草快氧化应激组和QG组腹腔注射等量敌草快(浓度为20 mg/kg BW)。结果显示,与对照组相比,敌草快氧化应激组肉鸡体重增长率和法氏囊指数显著下降(P<0.05),血清中免疫球蛋白A (IgA)、免疫球蛋白M (IgM)、免疫球蛋白G(IgG)和补体4 (C4)含量显著降低(P<0.05),肝脏中谷胱甘肽过氧化物酶(GSH-Px)活性和GSH-Px mRNA的表达量显著降低(P<0.05),丙二醛(MDA)含量显著升高(P<0.05)。与敌草快氧化应激组相比,QG组肉鸡的体重增长率和法氏囊指数显著升高(P<0.05),血清中IgA、IgG和补体3 (C3)的含量显著升高(P<0.05),肿瘤坏死因子-α (TNF-α)含量显著下降(P<0.05),肝脏中MDA含量显著降低(P<0.05)。研究表明,肉鸡饲粮中添加100 mg/kg QG可以缓解氧化应激导致的肉鸡体重降低,通过调节核因子E2相关因子2 (Nrf2)信号通路来改善抗氧化能力和提高机体免疫力。

The aim of this experiment was to investigate the effects of diet supplemented with quercetagetin(QG)on the antioxidant capacity and immune function of broiler chickens under diquat-induced oxidative stress.Two hundred and forty 1-day-old Cobb broilers were selected and randomly divided into a control group,a diquat induced oxidative stress(DOS)group,and a QG group,with eight replicates in each group and 10 broilers in each replicate.The control group and DOS group were fed a basal diet,and the QG group was fed a basal diet supplemented with 100 mg/kg QG.The experiment period was 23 d.One broiler close to the average body weight was selected from each replicate on the 20th day of the experiment.The control group was injected intraperitoneally with 1 mL of saline,and the DOS group and QG group were injected intraperitoneally with the same volume of diquat at a concentration of 20 mg/kg BW.The results showed that compared with the control group,the rate of body weight gain and bursal index in the DOS group significantly decreased(P<0.05),the concentrations of immunoglobulin A(IgA),immunoglobulin M(IgM),immunoglobulin G(IgG),and complement 4(C4)in the serum of the DOS group were significantly reduced(P<0.05),the glutathione peroxidase(GSH-Px)activity and expression of GSH-Px mRNA in the liver of boilers in the DOS group were significantly reduced(P<0.05),and malondialdehyde(MDA)content was significantly increased in the DOS group(P<0.05).Compared with the DOS group,the rate of body weight gain and bursal index in the QG group were significantly increased(P<0.05),the concentrations of immunoglobulin A(IgA),immunoglobulin G(IgG),and complement 3(C3)in the serum of the QG group significantly increased(P<0.05),and the MDA content was significantly reduced in the liver of boilers in the QG group (P<0.05). The study indicates that adding 100 mg/kg QGto broiler feed can alleviate the weight loss in broilers caused by oxidative stress, by modulating the Nrf2 signalingpathway to improve antioxidant capacity and enhance immune function.