饲料中添加高硒光合细菌对中华绒螯蟹抗氧化能力和食味品质的影响

Effects of adding high selenium photosynthetic bacteriato feed on antioxidant capacity and taste quality of Eriocheir sinensis

试验旨在探究高硒光合细菌在中华绒螯蟹(Eriocheir sinensis)池塘养殖中的应用效果。试验选用设施条件一致的养殖池6个,随机分为3组,试验蟹规格为(165±20) g/只。对照组投喂普通商品饲料,试验1组投喂1轮拌有高硒光合细菌培养物的试验饲料,试验2组投喂两轮含硒试验饲料,每轮连续4 d,有机硒日投喂量为130μg/kg蟹。结果显示,与对照组相比,两个试验组中华绒螯蟹肥满度、背甲红绿值(a*值)显著提高(P<0.05),血清、肝胰腺总抗氧化能力(T-AOC)显著提高(P<0.05),肝胰腺丙二醛(MDA)含量显著下降(P<0.05),肌肉和肝胰腺土腥味均显著减弱(P<0.05),肌肉鲜味显著增强(P<0.05)。与对照组相比,试验2组中华绒螯蟹肝胰腺和肌肉硒含量显著升高(P<0.05),肝胰腺a*值、黄蓝值(b*值)均显著升高(P<0.05);血清、肝胰腺超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)含量显著提高(P<0.05),血清MDA含量显著下降(P<0.05)。研究表明,投喂2轮试验饲料可显著提高中华绒螯蟹生长性能、肝胰腺与肌肉硒含量,提高机体抗氧化能力,改善色泽和可食部分风味。

The purpose of the study was to investigate the effect of high selenium photosynthetic bacteria(PSB)in the pond culture of Eriocheir sinensis.Six ponds with the same facilities were randomly divided into three groups.The size of test crab was(165±20)g/crab.The control group was fed with common commercial feed,the experimental group 1 was fed with one round of experimental feed mixed with high selenium photosynthetic bacteria culture,and the experimental group 2 was fed with two rounds of experimental feed containing selenium,each round lasted for four days,and the daily feeding amount of organic selenium was 130μg/kg crab.The results showed that compared with the control group,the fullness,red-green value of carapace(a*value),total antioxidant capacity(T-AOC)in serum and hepatopancreas of the two experimental groups increased significantly(P<0.05),malondialdehyde(MDA)content in hepatopancreas of the two experimental groups decreased significantly(P<0.05),the earthy smell of muscle and hepatopancreas of the two experimental groups decreased significantly(P<0.05),and the delicious taste of muscle of the two experimental groups was enhanced significantly(P<0.05).Compared with the control group,the content of selenium in hepatopancreas and muscle of Eriocheir sinensis in the experimental group 2 increased significantly(P<0.05),and the a*value and yellowblue value(b*value)of hepatopancreas of the experimental group 2 increased significantly(P<0.05),the activity of superoxide dismutase(SOD)and the content of glutathione(GSH)in serum and hepatopancreas of the experimental group 2 increased significantly(P<0.05),while the content of MDA in serum of the experimental group 2 decreased significantly(P<0.05).The study indicates that feeding two rounds of experimental feed can significantly improve the growth performance,selenium content in hepatopancreas and muscle,antioxidant capacity,color,and edible flavor of Eriocheir sinensis.