拮抗哈维氏弧菌的黄姑鱼肠道原籍益生菌的体外筛选

In vitro screening of autochthonous probiotics against Vibrio harveyi in yellow drum (Nibea albiflora)

试验旨在筛选对哈维氏弧菌具有防治作用的益生菌。试验通过比较红头病和健康黄姑鱼肠道微生物差异,进一步通过体外抑菌试验、药敏试验、生物安全性试验,筛选出对红头病病原哈维氏弧菌B0003具有拮抗作用且对宿主安全的肠道益生菌候选菌株。结果显示,红头病和健康黄姑鱼肠道微生物存在较大差异,选择12株候选菌株测试其对哈维氏弧菌B0003的拮抗作用。体外抑菌试验发现,5株候选菌对哈维氏弧菌B0003具有明显抑制作用,其抑菌能力由强到弱依次为:暹罗芽孢杆菌B0E14、贝莱斯芽孢杆菌DM5、巨大芽孢杆菌B1M2、枯草芽孢杆菌B0E9、粪肠球菌AT5。这5株拮抗菌均不具有溶血活性,且不会对黄姑鱼健康造成不利影响,对大多数抗菌药物敏感。研究表明,试验筛选的5株候选菌具有作为益生菌用于黄姑鱼红头病防治的潜力。

The purpose of the experiment was to screen probiotics with control effect on Vibrio harveyi.The experiment compared the differences in the gut microbiota between red-head disease N.albiflora and and healthy N.albiflora,and further in vitro bacteriostatic tests,drug sensitivity tests,and biosafety tests were conducted to screen autochthonous probiotic candidates that possess bacteriostatic ability on V.harveyi B0003 and were safe to N.albiflora.The results showed that intestinal microbiota between diseased and healthy N.albiflora showed huge differences,and 12 probiotic candidates were selected for antagonistic screening.Five strains,which could inhibit V.harveyi B0003,were screened by in vitro bacteriostatic tests,and their bacteriostatic ability was ranked from strong to weak in order as B.siamensis B0E14,B.velezensis DM5,B.megaterium B1M2,B.subtilis B0E9 and Enterococcus faecalis AT5.The five probiotic candidates did not have hemolytic activity,were non-harmful to N.albiflora,and were sensitive to many antibiotic drugs.The study indicates that the five antagonistic candidates have the potential to be used as probiotics for the prevention and treatment of red-head disease in N.albiflora aquaculture.