艾灸“心俞”“肺俞”对慢性心力衰竭大鼠心肌瞬时受体电位香草酸亚型1、降钙素基因相关肽表达的影响

Effect of moxibustion at“Xinshu”(BL15)and“Feishu”(BL13)on expression of TRPV1 and CGRP in the myocardial tissue of chronic heart failure rats

目的:观察艾灸“心俞”“肺俞”对慢性心力衰竭(CHF)大鼠心肌组织瞬时受体电位香草酸亚型1(TRPV1)、降钙素基因相关肽(CGRP)及血清白细胞介素-10(IL-10)的影响,探讨艾灸“心俞”“肺俞”治疗CHF的作用机制。方法:SD大鼠随机分为正常组、模型组、艾灸组、辣椒素组、艾灸+辣椒素组、艾灸+溶媒组,每组10只。采用冠状动脉左前降支结扎致心肌梗死法制备CHF大鼠模型。艾灸组每日艾灸“心俞”“肺俞”30 min;辣椒素组每日穴区涂抹辣椒素;艾灸+辣椒素组、艾灸+溶媒组分别将辣椒素、溶媒于艾灸前涂于穴区,艾灸方法同艾灸组。各组均治疗4周。采用小动物彩色多普勒超声仪检测各组大鼠射血分数(EF)及左室短轴缩短率(FS);HE染色法观察大鼠心肌细胞形态结构;实时荧光定量PCR和Western blot法检测大鼠心肌组织中TRPV1、CGRP、半乳糖凝集素-3(Gal-3)mRNA和蛋白表达水平;ELISA法检测大鼠血清IL-10含量。结果:与正常组比较,模型组大鼠心肌纤维紊乱,炎性细胞浸润明显,EF及FS降低(P<0.01),心肌TRPV1、CGRP mRNA和蛋白表达水平及血清IL-10含量降低(P<0.01),心肌Gal-3 mRNA和蛋白表达水平升高(P<0.01)。与模型组比较,艾灸组、辣椒素组、艾灸+辣椒素组上述指标均逆转(P<0.01)。与艾灸组、辣椒素组比较,艾灸+辣椒素组效果更佳(P<0.05,P<0.01)。结论:艾灸“心俞”“肺俞”可以改善CHF大鼠心肌损伤,其作用可能与调控TRPV1通路,升高TRPV1、CGRP mRNA和蛋白表达,促进抗炎因子IL-10上调,降低Gal-3 mRNA和蛋白表达水平,从而减轻心肌纤维化有关。

Objective To observe the effect of moxibustion at“Xinshu”(BL15)and“Feishu”(BL13)on transient receptor potential vanilloid type 1(TRPV1),calcitonin gene-related peptide(CGRP),and serum interleukin-10(IL-10)in the myocardial tissue of rats with chronic heart failure(CHF),so as to explore its underlying mechanisms in improvement of CHF.Methods Male SD rats were randomly divided into the normal,model,moxibustion,capsaicin,moxibustion+capsaicin,and moxibustion+solvent groups,with 10 rats in each group.The CHF model was established by permanent ligation of the anterior descending branch of the left coronary artery.Mild moxibustion was applied to bilateral BL13 and BL15 for 30 min once daily for 4 weeks.Rats in the capsaicin group were smeared with capsaicin in the acupoint area once a day for 4 weeks.For rats of the moxibustion+capsaicin and moxibustion+solvent groups,capsaicin and solvent were applied to the acupoint area before moxibustion for 4 weeks,respectively.The ejection fraction(EF)and left ventricular fractional shortening rate(FS)were examined by echocardiography.HE staining was used to observe the myecardial morphological structure.The mRNA and protein expression levels of TRPV1,CGRP and galectin-3(Gal-3)in myocardial tissue were detected by real-time quantitative PCR and Western blot,respectively.The content of IL-10 in serum was detected by ELISA.Results After modeling,the pathological changes of myocardium(as cardiac muscle fiber disorder,inflammatory cell infiltration,etc.)were obvious,and the EF,FS,serum IL-10,protein and mRNA exspression of TRPV1 and CGRP were significantly decreased(P<0.01)in the model group compared with the normal group,while the protein and mRNA exspression of Gal-3 were significantly up-regulated(P<0.01).Following the interventions,the above-mentioned indexes were all reversed in moxibustion,capsaicin,and moxibustion+capsaicin groups(P<0.01),and the effect of moxibustion+capsaicin was the best(P<0.05,P<0.01).Conclusion Moxibustion can reduce myocardial injury and improve cardiac function in CHF rats,which may be related to its effects in up-regulating the expression of TRPV1 and CGRP,and down-regulating the expression of Gal-3 to alleviate myocardial fibrosis.