玉米shrunken2突变体中醇溶蛋白合成相关基因的表达分析

Expression Analysis of Zein Synthesis-related Gene in Near Isogenic Line of shrunken2 in Maize

利用SDS-PAGE和荧光定量PCR,研究shrunken2(sh2)突变体子粒胚乳发育不同时期醇溶蛋白的积累以及醇溶蛋白合成相关基因的表达情况。结果表明,与郑58相比较,sh2中氨基酸组分变化与opaque2(o2)相似,赖氨酸含量升高,同时谷氨酸和亮氨酸含量下降。SDS-PAGE结果显示,胚乳发育不同时期sh2中醇溶蛋白的积累均明显低于郑58,而且sh2中19-kDaα-醇溶蛋白积累明显减少。荧光定量PCR结果表明,sh2突变体中对于Z1A、Z1B和Z1D家族基因的抑制较为显著。sh2突变体中prolamine-box binding factor(PBF)表达量升高,opaque2(o2)表达下降。因此,sh2突变体同时影响淀粉、氨基酸、贮藏蛋白等的代谢。

SDS-PAGE and fluorescent quantitative PCR were used to investigate the accumulation of zein and the expression of genes related to their synthesis during different stages of endosperm development in shrunken2(sh2)mutant kernels.The study found that the changes in amino acid composition in sh2 were similar to those in opaque2(o2),with an increase in lysine content and a decrease in glutamic acid and leucine content compared to Zheng58.SDS-PAGE results showed that the accumulation of zein in sh2 at different stages of endosperm development was significantly lower than that in Zheng58,and the accumulation of 19-kDaα-zeins in sh2 was significantly reduced.The results of fluorescent quantitative PCR showed that the inhibition of the Z1A,Z1B,and Z1D gene families was more significant in the sh2 mutant.The expression of prolamine-box binding factor(PBF)was increased in the sh2 mutant,while that of opaque2(o2)was decreased.Therefore,sh2 mutation affects the metabolism of starch,amino acids,and storage proteins.