单宁酸对玉米赤霉烯酮致小鼠肝脏和肾脏氧化损伤的修复作用

Repair effects of tannic acid on zearalenone-induced oxidative damage of liver and kidney in mice

为了探究单宁酸(TA)对玉米赤霉烯酮(ZEA)中毒小鼠肝脏和肾脏损伤的修复作用,试验将75只健康雌性昆明小鼠随机分为5组,即对照组、ZEA(10 mg/kg)组和TA低、中、高剂量(50,100,200 mg/kg)组,每组15只。对照组灌服10%乙醇溶液,其余组均灌服ZEA(溶于10%乙醇),每天1次,连续7 d;之后除对照组和ZEA组灌服双蒸水外,TA组分别灌服不同剂量的TA,连续7 d。采集肝脏和肾脏,计算脏器指数,经苏木精-伊红染色后观察肝脏和肾脏的病理变化,检测肝脏和肾脏组织中活性氧(ROS)、丙二醛(MDA)、还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果表明:与对照组相比,ZEA组肝脏和肾脏指数无显著变化(P>0.05);肝细胞脂肪变性,伴有炎症细胞浸润,且部分肝细胞出现核固缩,同时中央静脉内红细胞和血浆沉积;肾脏皮质和髓质出现大量红细胞,肾小球和肾小管上皮细胞出现肿胀;肝脏中ROS和GSH含量显著或极显著增加(P<0.05或P<0.01),SOD活性极显著升高(P<0.01)。与ZEA组相比,TA各剂量组肝脏和肾脏形态结构损伤减轻。在肝脏,TA各剂量组ROS含量显著或极显著降低(P<0.01或P<0.05),TA低剂量组SOD和GSH-Px活性显著或极显著升高(P<0.05或P<0.01),TA各剂量量组GSH含量显著或极显著增加(P<0.05或P<0.01);在肾脏,TA低剂量组GSH含量显著增加(P<0.05),TA中、高剂量GSH-Px活性极显著降低(P<0.01)。说明TA通过恢复肝脏和肾脏形态结构维持氧化还原系统平衡,缓解ZEA造成的肝脏和肾脏氧化损伤,从而发挥保护作用。

In order to investigate the repair effects of tannic acid(TA)on zearalenone(ZEA)-induced oxidative damage of liver and kidney in mice,in the experiment,seventy-five healthy female Kunming mice were randomly divided into the control group,ZEA(1O mg/kg)group,and TA low,medium,and high dose(50,100,and 200 mg/kg)groups,with 15 mice in each group.The control group was irrigated with 10%ethanol solution,and the rest of the groups were irrigated with ZEA(dissolved in 10%ethanol),once a day for 7 d.After that,except for control group and ZEA group which were irrigated with double-distlled water,the TA group was irrigated with different doses of TA for 7 consecutive days.Liver and kidney were collected and organ index was calculated;hematoxylin-eosin staining was performed to observe the pathological changes of liver and kidney;the contents of reactive oxygen species(ROS),malondialdehyde(MDA),reduced glutathione(CSH)and the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(CSH-Px)were examined in the liver and kidney tissues.The results showed that compared with the control group,there was no significant change in liver and kidney index in the ZEA group(P>0.05);hepatocyte steatosis,accompanied by inflammatory cell infiltration,nuclear condensation of some hepatocytes,and deposition of red blood cells and plasma in the central vein appeared.A large number of red blood cells appeared in the renal cortex and medulla,and the glomerular and renal tubular epithelial cells were swollen;the contents of ROS and CSH in the liver were significantly or extremely significantly increased(P<0.05 or P<0.01),and the activity of SOD was extremely significantly increased(P<0.01).Compared with the ZEA group,the morphological and structural damage of liver and kidney was reduced in the TA dose group.In liver,the content of ROS in each TA dose group was significantly or extremely significantly decreased(P<0.05 or P<0.O1);the activities of SOD and CSH-Px in the TA low dose group were significantly or extremely significantly increased(P<0.05 or P<0.01);the GSH content in the TA dose group was significantly or extremely significantly increased(P<0.05 or P<0.01).In the kidney,the GSH content in the low-dose TA group was significantly increased(P<0.05),and the activity of GSH-Px in the medium and high dose TA groups was extremely significantly decreased(P<O.O1).These results indicated that TA played a protective role by restoring the morphological structure of the liver and kidney,maintaining the balance of the redox system,and alleviating the oxidative damage of the liver and kidney caused by ZEA.