超声辅助分散液液微萃取-气相色谱-质谱法测定玫瑰花水中10种香气组分的含量

Determination of 10 Aroma Components in Rose Water by Gas Chromatography-Mass Spectrometry with Ultrasound Assisted Dispersive Liquid-Liquid Microextraction

提出了超声辅助分散液液微萃取-气相色谱-质谱法测定玫瑰花水中香气组分芳樟醇、乙酸香茅酯、乙酸香叶酯、香茅醇、橙花醇、香叶醇、苯甲醇、苯乙醇、甲基丁香酚、丁香酚含量的方法。取7.0 mL玫瑰花水样品,加入含400μL乙腈、200μL二氯甲烷的分散微萃取溶液,涡旋1 min,超声3 min,离心3 min,收集全部下层沉淀相,注入气相色谱-质谱仪。以HP-INNOWAX色谱柱为固定相,在程序升温条件下分离10种香气组分,以电子轰击离子源电离,外标法定量。结果显示:10种香气组分的质量浓度均在一定范围内和峰面积呈线性关系,检出限(3S/N)为0.050~0.078 mg·L^(−1)。按照标准加入法进行回收试验,回收率为79.8%~114%,测定值的相对标准偏差(n=6)为2.1%~9.6%。

A method was proposed for the determination of aroma components of linalool,citronellol acetate,geranyl acetate,citronellol,nerol,geraniol,benzyl alcohol,phenethyl alcohol,methyl eugenol,and eugenol in rose water by gas chromatographymass spectrometry with ultrasound assisted dispersive liquid-liquid microextraction.The 7.0 mL of rose water sample was taken,and the dispersive microextraction solution containing 400μL of acetonitrile and 200μL of dichloromethane was added.After vortex for 1 min,ultrasound for 3 min and centrifugation for 3 min,all lower precipitates were collected,and injected the gas chromatograph-mass spectrometer.Using HP-INNOWAX chromatographic column as the stationary phase,10 aroma components were separated under programmed temperature conditions,ionized by electron impact ion source,and quantified by the external standard method.It was shown that linear relationships between values of the mass concentration and the peak area of 10 aroma components were kept in definites ranges,with detection limits(3S/N)in the range of 0.050-0.078 mg·L^(−1).According to the standard addition method,test for recovery was made,giving recoveries in the range of 79.8%-114%,and RSDs(n=6)of the determined values in the range of 2.1%-9.6%.