阿霉素对SD大鼠肠道真菌的影响

Effect of adriamycin on gut fungi in SD rats

目的探究阿霉素处理后SD大鼠肠道真菌的改变。方法14只SD大鼠随机分为对照组和阿霉素组,阿霉素组采用阿霉素(7.5 mg/kg)单次尾静脉注射,6周后收集大鼠粪便进行ITS2测序及生物信息学分析,比较两组大鼠肠道真菌丰度及多样性变化,并进行差异菌属的鉴定。结果ITS2测序结果显示阿霉素组大鼠肠道真菌的丰度和多样性与对照组比较,差异无统计学意义(P>0.05),Beta多样性差异有统计学意义(P<0.05)。在肠道真菌门水平,子囊菌门、unclassified-Fungi、unclassified、担子菌门、黏液菌门、蜕皮菌门占主导地位;在科水平,以白粉菌科、unclassified-Fungi、unclassified、孢菌科、曲霉科等为优势微生物;在属水平相对丰度由高到低依次为白粉病菌属、unclassified-Fungi、unclassified、链格孢属、曲霉属等。LEfSe结果显示阿霉素处理后,在属水平,unclassified-Fungi、丝衣霉属、链格孢菌属、金孢子菌属等13种真菌相对丰度显著减少,而木霉菌属、隐球菌属、毛霉属和白粉病菌属丰度显著增高。结论SD大鼠经阿霉素处理后,肠道真菌的菌群结构发生显著改变,不同分类水平下部分真菌的相对丰度存在明显变化。

Objective To investigate the effect of adriamycin on gut fungi in SD rats.Methods Fourteen SD rats were randomly divided into control and adriamycin groups,rats in adriamycin group were administered with adriamycin 7.5 mg/kg by single tail vein injection.The rat feces were collected for ITS2 sequencing and bioinformatics analysis after 6 weeks.The gut fungal abundance and diversity were compared between two groups and the differential genera were identified.Results ITS2 sequencing results showed that there were no significant differences in abundance and diversity of gut fungi between adriamycin group and control group(P>0.05),while Beta diversity varied significantly(P<0.05).The composition of gut fungi:at the phylum level Ascomycota,unclassified-Fungi,unclassified,Basidiomycota,Chytridiomycota and Glomeromycota were predominant;at the family level Erysiphaceae,unclassified-Fungi,unclassified,Sporidiobolaceae,and Aspergillaceae were predominant;at the genus level,the relative abundance from high to low was Erysiphe,unclassified-Fungi,unclassified,Cladosporium,Aspergillus,etc.The LEfSe results revealed that the relative abundance of 13 fungi including unclassified-Fungi,Byssochlamys,Alternaria,Chrysosporium decreased significantly at the genus level after adriamycin treatment,while the relative abundance of Trichoderma,Cryptococcus,Actinomucor,and Blumeria increased significantly.Conclusion The diversity of rat gut fungi is changed significantly after adriamycin treatment and the abundance of certain gut fungi is changed at different taxonomic levels.