右侧星状神经节阻滞对后肢缺血再灌注诱发大鼠急性肺损伤的影响

Effect of right stellate ganglion block on acute lung injury induced by hind limb ischemia-reperfusion in rats

目的:评价星状神经节阻滞(SGB)对后肢缺血再灌注诱发大鼠急性肺损伤的影响。方法:SPF级健康雄性SD大鼠27只,7~8周龄,体质量200~250 g,采用随机数字表法分为3组(n=9):假手术组(Sham组)、后肢缺血再灌注组(I/R组)和右侧SGB+后肢缺血再灌注组(SGB+I/R组)。I/R组和SGB+I/R组制备右后肢缺血再灌注损伤模型,SGB+I/R组右后肢缺血前,采用0.2%罗哌卡因0.5 ml行右侧SGB。光镜下观察肺组织病理学结果;测定肺组织湿质量/干质量比值、PaO_(2)和PaCO_(2),计算氧合指数;采用ELISA法测定血清丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)的浓度。结果:与Sham组相比,I/R组和SGB+I/R组肺组织湿质量/干质量比值、PaO_(2)、氧合指数和GSH-Px浓度降低,血清MDA浓度升高(P<0.05);与I/R组相比,SGB+I/R组肺组织湿质量/干质量比值、PaO_(2)、氧合指数和血清GSH-Px浓度升高,血清MDA浓度降低(P<0.05),肺组织病理学损伤减轻。3组PaCO_(2)比较差异无统计学意义(P>0.05)。结论:右侧SGB可减轻后肢缺血再灌注诱发大鼠急性肺损伤,其机制可能与抑制氧化应激反应有关。

Objective To evaluate the effect of the right stellate ganglion block(SGB)on the acute lung injury induced by hind limb ischemia-reperfusion(I/R)in rats.Methods Twenty-seven SPF healthy male Sprague-Dawley rats,aged 7-8 weeks,weighing 200-250 g,were divided into 3 groups(n=9 each)by the random number table method:sham operation group(Sham group),hind limb I/R group(I/R group)and right SGB+hind limb I/R group(SGB+I/R group).The right hind limb I/R injury model was developed in anesthetized rats in I/R and SGB+I/R groups,and the right SGB was performed with 0.2%ropivacaine 0.5 ml before the right hind limb ischemia in SGB+I/R group.The pathological changes of lung tissues were observed with a light microscope,the wet/dry lung weight ratio,PaO_(2) and PaCO_(2) were determined,and the oxygenation index was calculated.The concentrations of serum malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)were determined using enzyme-linked immunosorbent assay.Results Compared with Sham group,the wet/dry lung weight ratio,PaO_(2),oxygenation index,and GSH-Px concentrations were significantly decreased,and the concentrations of serum MDA were increased in I/R and SGB+I/R groups(P<0.05).Compared with I/R group,the wet/dry lung weight ratio,PaO_(2),oxygenation index,and GSH-Px concentrations were significantly increased,the concentrations of serum MDA were decreased(P<0.05),and the pathological changes of lung tissues were significantly attenuated in SGB+I/R group.There was no significant difference in PaCO_(2) among the 3 groups(P>0.05).Conclusions The right SGB can alleviate the acute lung injury induced by hind limb I/R,and the mechanism may be related to the inhibition of oxidative stress responses in rats.