负载姜黄素-Ag^(+)的聚多巴胺复合水凝胶减轻大鼠颌下腺辐射损伤及抗菌性能分析

Polydopamine hydrogel loaded with curcumin-Ag^(+)in alleviating radiation damage in rat submandibular glands and its antibacterial properties analysis

目的:合成负载姜黄素(Cur)-Ag^(+)的聚多巴胺(PDA)复合水凝胶,探究其降低放疗引发的唾液腺细胞放射性损伤的效果,及复合水凝胶中Cur、Ag^(+)的协同抑菌作用。方法:合成负载Cur-Ag^(+)的复合水凝胶,对其进行表征。将不同浓度的Cur及负载不同浓度Cur-Ag^(+)的复合水凝胶与人鼻咽癌细胞系(5-8F细胞)、SD大鼠颌下腺原代细胞共培养,通过细胞计数试剂盒(CCK-8)实验检测细胞活力;将负载不同浓度Cur-Ag^(+)的水凝胶与SD大鼠颌下腺原代细胞共孵育,利用电子直线加速器对其进行不同剂量(0 Gy、5 Gy、10 Gy、15 Gy)的X线照射,72 h后通过CCK-8实验检测各组细胞活力。将PDA胶及负载不同浓度Cur-Ag^(+)的复合水凝胶与大肠杆菌、金黄色葡萄球菌共培养后,检测12 h内细菌生长曲线;48 h后观察琼脂平皿内抑菌情况;将不同浓度Cur、Ag^(+)与两种细菌共培养24 h后检测细菌生物膜生长情况。结果:负载Cur-Ag^(+)的PDA复合水凝胶在实验浓度下具备良好的生物兼容性,孵育72 h后,大鼠颌下腺原代细胞活性均在80%以上,并具备一定程度的肿瘤生长抑制作用;大鼠颌下腺原代细胞接受5 Gy、10 Gy、15 Gy放射剂量照射后,相比未经处理的纯照射组细胞,与复合水凝胶共孵育后的细胞其活性可提高30%;保持培养基中Ag^(+)浓度为0.1μg/mL固定不变,随着Cur浓度升高,生物膜生长抑制作用逐渐增强;生长曲线及平板抑菌结果也展现出明显的协同抗菌效果。结论:负载Cur-Ag^(+)的PDA复合水凝胶,可有效降低放疗引发的唾液腺细胞放射性损伤,同时,复合水凝胶中Cur、Ag^(+)可起到协同抑菌的作用。

Objective:To synthesize composite hydrogels loaded with curcumin(Cur)−Ag^(+)using polydopamine(PDA)hydrogel,and to investigate their effect on mitigating radiation-induced damage to salivary gland cells,as well as the synergistic antibacterial effect of Cur and Ag^(+)in the composite hydrogels.Methods:The synthesis of omposite hydrogels loaded with Cur−Ag^(+)and characterization observation were performed.Different concentrations of Cur and composite hydrogels loaded with varying concentrations of Cur−Ag^(+)were co−cultured with human nasopharyngeal carcinoma squamous cells(5-8F cells)and primary submandibular gland cells of SD rats.Cell viability was assessed by cell counting kit-8(CCK-8)assay.Composite hydrogels loaded with different concentrations of Cur-Ag^(+)were incubated with SD rat submandibular gland primary cells and exposed to different doses of X-ray irradiation(0 Gy,5 Gy,10 Gy,15 Gy)using an electron linear accelerator.The cell viability of each group was evaluated using the CCK-8 assay 72 hours later.PDA glue and various concentrations of composite hydrogels were co-cultured with Escherichia coli and Staphylococcus aureus,and the bacterial growth curve was monitored over 12 hours.Bacteriostasis on AGAR plates was observed after 48 hours.The growth of biofilm was assessed after co-culturing different concentrations of Cur and Ag^(+)with the two types of bacteria for 24 hours.Results:The composite hydrogels exhibited favorable biocompatibility at the experimental concentrations.After incubation for 72 hours,the activity of primary submandibular gland cells was above 80%,and demonstrated a certain cytotoxic effect against tumor cells.After 5 Gy,10 Gy,15 Gy irradiation,the activity of primary submandibular gland cells of rats after co-incubation with complex hydrogel could be increased by 30%compared with the untreated pure irradiation group.The concentration of Ag^(+)in the medium was kept constant at 0.1μg/mL,and the inhibitory effect of biofilm growth was gradually enhanced with the increase of Cur concentration.The growth curve and plate antibacterial results also showed obvious synergistic antibacterial effect.Conclusion:PDA composite hydrogels loaded with CUR-Ag^(+)can effectively reduce the radiation-induced damage to salivary gland cells.Among the composite hydrogels,the combined application of Cur and Ag^(+)demonstrates enhanced synergistic antibacterial properties.