阿苯达唑对人食管鳞癌细胞KYSE-150的抑制作用

Inhibitory Effect of Albendazole on Human Esophageal Squamous Cell Carcinoma Cells KYSE-150

目的研究阿苯达唑对食管鳞癌KYSE-150细胞的抑制效果及作用机制。方法通过MTT法和克隆形成实验评估KYSE-150细胞的增殖能力,利用划痕和Transwell小室实验证明细胞的迁移和侵袭能力。同时,采用凋亡与坏死检测试剂盒检测阿苯达唑是否能够诱导KYSE-150细胞凋亡,并利用Western blot实验探讨凋亡相关蛋白BAX、Bcl-2、P53的表达水平。结果阿苯达唑能够以剂量依赖性的方式抑制KYSE-150细胞的活力、增殖、迁移和侵袭能力。YO-PRO-1/PI双染检测结果表明,阿苯达唑能够诱导细胞凋亡,同时使Bcl-2蛋白表达下调,而BAX、P53蛋白表达上调。结论阿苯达唑对食管鳞癌KYSE-150细胞的增殖与迁移具有显著抑制作用,其机制可能与对凋亡相关蛋白的调控有关。

Objective To investigate the inhibitory effect and mechanism of albendazole on esophageal squamous cell carcinoma KYSE-150 cells.Methods The proliferation ability of KYSE-150 cells was evaluated by MTT assay and colony formation assay.The migration and invasion ability of KYSE-150 cells were demonstrated by wound healing assay and Transwell chamber assay.Meanwhile,the apoptosis and necrosis detection kit was used to detect whether albendazole could induce apoptosis of KYSE-150 cells,and the expression levels of apoptosis-related proteins BAX,Bcl-2 and P53 were investigated by Western blot.Results Albendazole inhibited the viability,proliferation,migration and invasion of KYSE-150 cells in a dose-dependent manner.The results of YO-PRO-1/PI double staining showed that the administration could induce cell apoptosis,and at the same time,it caused the down-regulation of the expression of Bcl-2 protein,while the up-regulation of the expression of BAX and P53 protein.Conclusion Albendazole has a significant inhibitory effect on the proliferation and migration of esophageal squamous carcinoma KYSE-150 cells,and the mechanism may be related to the regulation of apoptosis-related proteins.