番石榴叶总黄酮对高脂血症大鼠血脂、脂代谢酶及血液流变学的影响

The Influence of Total Flavonoids of Guava Leaves on Blood Lipid and Lipid Metabolism Enzyme and Hemorheology in Rats with Hyperlipidemia

目的:观察番石榴叶总黄酮对脂肪乳剂所致高脂血症大鼠血脂、脂代谢酶及血液流变学的影响。方法:采用脂乳剂灌胃法建立大鼠高脂血症模型,将50只大鼠随机分为空白组、模型组、阿托伐他汀钙片(3.3 mg·kg^(-1))组、番石榴叶总黄酮高剂量(200 mg·kg^(-1))组、番石榴叶总黄酮低剂量(100 mg·kg^(-1))组,每组各10只,连续给药6周。检测血清中三酰甘油(triglyceride,TG)、总胆固醇(triglyceride,TC)、高密度脂蛋白胆固醇(high density lipid protein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid protein cholesterol,LDL-C)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙二醛(malondialdehyde,MDA)、总抗氧化能力(total antioxidative capability,T-AOC)、谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine transaminase,ALT)、碱性磷酸酶(alkaline phosphatase,ALP)水平,同时检测全血及血浆的血液流变学相关指标。结果:与模型组比较,番石榴叶总黄酮高剂量组、番石榴叶总黄酮低剂量组及阿托伐他汀钙片组均可不同程度降低大鼠血清中TC、TG、LDL-C、MDA、AST、ALT、ALP水平,升高HDL-C、SOD、GSX-PX、T-AOC水平,降低血液流变学各项指标(P<0.05)。结论:番石榴叶总黄酮可有效调节高脂血症大鼠血脂水平,提高脂代谢酶活性,改善血液流变学指标,有效防治高脂血症。

Objective:To observe the influence of total flavonoids of guava leaves on blood lipid and lipid metabolism Eenzyme and hemorheology in rats with hyperlipidemia induced by fat emulsion.Methods:The hyperlipidemia rat model was established by intragastric administration of fat emulsion.A total of 50 rats were randomly divided into the blank group,the model group,the atorvastatin calcium tablet(3.3 mg·kg^(-1))group,the total flavonoids of guava leaves high-dose(200 mg·kg^(-1))group and the total flavonoids of guava leaves low-dose(100 mg·kg^(-1))group,with 10 rats in each group for 6 weeks.Then the levels of serum triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),superoxide dismutase(SOD),were detected glutathione peroxidase(GSH-Px),malondialdehyde(MDA),total antioxidant capacity(T-AOC),aspartate aminotransferase(AST),alanine transaminase(ALT)and alkaline phosphatase(ALP)were detected,At the same time,the hemorheological indexes of whole blood and plasma were also detected.Results:Compared with the model group,the levels of TC,TG,LDL-C,MDA,AST,ALT,ALP in the total flavonoids of guava leaves high-dosegroup,the total flavonoids of guava leaves low-dose group,and the atorvastatin calcium tablet group were decreased,while the levels of HDL-C,SOD,GSX-PX and T-AOC can be increased,and the indexes of hemorheology can be reduced(P<0.05).Conclusion:total flavonoids of guava leaves can effectively regulate the level of blood lipid,improve the activity of lipid metabolism enzymes,improve the indexes of hemorheology,and effectively prevent and treat hyperlipidemia.