西洋参红景天复配方对氧化损伤模型小鼠的抗氧化作用及机制

Antioxidant Effect and Mechanism of American GinsengRhodiola Formula on Oxidative Damage Model Mice

目的 评价西洋参红景天复配方对乙醇诱导的氧化损伤模型小鼠的抗氧化作用,并探讨其作用机制,为保健食品的开发提供科学依据。方法 小鼠按体质量随机均分为空白对照组、急性氧化损伤模型组、西洋参红景天复配方低剂量(330 mg/kg)、中剂量(660 mg/kg)、高剂量(990 mg/kg)组,每组10只,各剂量组经口灌胃给予受试样品,空白对照组和模型组给予等容量蒸馏水,连续灌胃30 d后,除空白对照组外各组禁食16 h后按12 mL/kg灌胃给予50%乙醇,6 h后取小鼠血、肝。检测小鼠血清的丙二醛(MDA)、8-表氢氧异前列腺素(8-Isoprostane)、蛋白质羰基(PCO)、谷胱甘肽(GSH)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、甘油三酯(TG)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,肝脏组织的MDA、PCO、GSH含量和SOD活性,利用蛋白印迹方法检测p-p38,p-ERK和p-JNK的蛋白表达。结果 与模型组相比较,西洋参红景天复配方低剂量组小鼠血清中MDA含量下降(P<0.05),各剂量组8-Isoprostane、PCO含量均降低(P<0.05),各剂量组GSH含量及GSH-Px、SOD活力均上升(P<0.05),各剂量组TNF-α、IL-1β、IL-6水平均降低(P<0.05),各剂量组ALT、AST活力下降(P<0.05)。低中剂量组小鼠肝脏中MDA含量下降(P<0.05),各剂量组PCO、TG含量均下降(P<0.05),低中剂量组SOD活性和低剂量组GSH含量上升(P<0.05)。低剂量组能抑制p-p38和p-JNK的表达(P<0.05),中高剂量组能抑制p-p38、p-ERK和p-JNK的表达(P<0.05)。结论 以红景天、西洋参、针叶樱桃粉及葡萄籽提取物组成的药食同源复配方具有较强的抗氧化保健作用。

Objective To evaluate the antioxidant effect of American ginseng Rhodiola formula on ethanol-induced oxidative d amage in model mice,and to explore its mechanism of action,providing a scientific basis for the development of healthy food.Methods Mice were randomly divided into blank control group,acute oxidative injury model group,low dose(330 mg/kg),medium dose(660 mg/kg)and high dose(990 mg/kg)groups according to body weight,10 mice in each group.Each dose group was given test samples by oral gavage.Blank control group and model group were given equal volume of distilled water.After continuous gavage for 30 days,50%ethanol was intragastrically administered at 12 mL/kg after fasting for 16 h in each group except blank control group.Blood and liver were collected from mice 6 h later.Malondialdehyde(MDA),8-epoxyisoprostane(8-Isoprostane),protein carbonyl(PCO),glutathione(GSH),aspartate aminotransferase(AST),alanine aminotransferase(ALT),triglyceride(TG),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)content and superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity in mouse serum,MDA,PCO,GSH content and SOD activity in liver tissue were measured,and the protein expression of p-p38,p-ERK and p-JNK was detected by Western blotting..Results Compared with the model group,the MDA content in the serum of mice in the low-dose group of American ginseng Rhodiola formula decreased(P<0.05),the 8-Isoprostane and PCO contents in each dose group decreased(P<0.05),the GSH content and GSH-Px and SOD activities in each dose group increased(P<0.05),the TNF-α,IL-1β,and IL-6 levels in each dose group decreased(P<0.05),and the ALT and AST activities in each dose group decreased(P<0.05).The MDA content in the liver of mice in the low and medium dose groups decreased(P<0.05),the PCO and TG contents in each dose group decreased(P<0.05),and the SOD activity in the low and medium dose groups and the GSH content in the low dose group increased(P<0.05).The low dose group could inhibit the expression of p-p38 and p-JNK(P<0.05),and the medium and high dose groups could inhibit the expression of p-p38,p-ERK and p-JNK(P<0.05).Conclusion Rhodiola rosea,American ginseng,acerola cherry powder and grape seed extract composition of medicinal food homologous complex formula has a strong antioxidant health care effect.