奶牛小肠上皮细胞系细胞染色体核型与G-带分析

Chromosome Karyotype and G-band Analysis of Bovine Small Intestinal Epithelial Cell Line

为了鉴定奶牛小肠上皮细胞系(BIECs-21)的生物学遗传稳定性,体外贴壁培养BIECs-21细胞,制备染色体标本和G-带标本,分析BIECs-21细胞的染色体核型和G-带。结果表明:BIECs-21细胞染色体数为2n=60,包括常染色体29对和性染色体(X和Y染色体)1对。其中,29对常染色体均为端部着丝点染色体;性染色体中,X染色体是较大的中着丝粒染色体,Y染色体是较小的中着丝粒染色体。除X、Y性染色体外,BIECs-21细胞常染色体的G-带带纹的数量和位置无显著差异。BIECs-21细胞与荷斯坦牛的正常染色体核型相比,染色体数目及形态结构均没有明显变化,证明该细胞系遗传特性稳定,可以用于牛肠道相关疾病分子机制的研究。

In order to identify the genetic stability in biology of bovine small intestinal epithelial cell line(BIECs-21),BIECs-21 cells were cultured in vitro by using adherent culture,and chromosome and G-band samples were prepared to analyze the chromosome karyotype and G-band of BIECs-21 cells.The results show that the number of chromosomes in BIECs-21 cells is 2n=60,including 29 pairs of autosomes and 1 pair of sex chromosomes(X and Y chromosomes).Among them,29 pairs of autosomes are all terminal centromere chromosomes,with X chromosome being the larger centromere chromosome and Y chromosome being the smaller centromere chromosome in the sex chromosome.There is no significant difference in the number and position of G-bands pattern on the autosomes of BIECs-21 cells,except for X and Y sex chromosome.Compared with the normal chromosome karyotype of Holstein cattle,there is no significant change in the number and morphological structure of chromosomes in BIECs-21 cells,indicating that the genetic characteristics of the cell line are stable,and it can be used for the reaearch on the molecular mechanisms of bovine intestinal related diseases.