miRNA-200b在糖尿病小鼠海马体中的异常表达及靶基因预测

Abnormal Expression of miRNA-200b in Hippocampus of Diabetes Mice and Prediction of Target Genes

目的分析糖尿病小鼠海马体miRNA-200b表达情况,并预测miRNA-200b靶基因。方法采用链脲佐菌素或联合高糖高脂饮食分别诱导1型和2型糖尿病小鼠模型,建模后处死小鼠,分离海马体组织,利用荧光定量PCR测定miRNA-200b表达量。通过miRWalk、miRDB和miRPathDB三个基因数据库预测miRNA-200b靶基因,进行GO分析和KEGG分析。结果成功建立糖尿病小鼠模型。糖尿病小鼠海马体中miRNA-200b表达量比正常小鼠下降(P<0.05)。生物信息学预测显示,miRNA-200b筛选到3个靶基因,分别是GAB1、SLC5A3和GICCL1,靶基因功能富集于糖代谢、脑部神经营养等信号通路。结论miRNA-200b在糖尿病小鼠海马体中显著下降,有望成为糖尿病治疗的新靶点。

Objective To analyze the expression of miRNA-200b in hippocampus of diabetes mice and predict the target gene of miRNA-200b.Methods Type 1 and type 2 diabetes mouse models were induced by streptozotocin or combined with high-sugar and high-fat diet,respectively.After modeling,the mice were killed and the hippocampus tissues were isolated.The expression of miRNA-200b was detected by fluorescence quantitative PCR.miRNA-200b target genes were predicted by miRWalk,miRDB and miRPathDB gene databases.Then,GO analysis and KEGG analysis were performed.Results The diabetes mouse model was successfully established,and the expression levels of miRNA-200b in the hippocampus of diabetic mice were decreased compared with that of normal mice(P<0.05).Bioinformatics prediction showed that three target genes of miRNA-200b were screened,namely GAB1,SLC5A3 and GICCL1.The functions of target genes were concentrated in the signaling pathways of glucose metabolism and neuronutrition.Conclusion The results of this study suggest that miRNA-200b is significantly decreased in the hippocampus of diabetic mice,which is expected to be a new target for diabetes treatment.