水晶兰苷对双侧卵巢摘除诱导骨质疏松模型大鼠的作用研究

Effect of Monotropein on Osteoporosis Induced by Bilateral Ovariectomy in Rats

目的研究巴戟天提取物水晶兰苷对绝经后骨质疏松模型大鼠的治疗作用。方法采用随机分组方法,将SD大鼠平均分为正常组、模型组、E2组、水晶兰苷组和巴戟天组。通过双侧性腺摘除法建立绝经后骨质疏松模型,给药组分别给予水晶兰苷和巴戟天灌胃。十二周后给药结束,解剖大鼠,之后测定血清生化指标、股骨组织形态及相关指标和氧化应激指标。结果与正常组比较,模型组大鼠血清中钙、磷、碱性磷酸酶、骨钙素和成骨特异性转录因子的水平升高,骨小梁数量减少,骨体积分数、骨表面积骨体积比、骨小梁厚度降低,骨小梁分离度增加。而水晶兰苷、巴戟天和17β雌二醇(E2)给药干预后,大鼠骨质疏松血清关键指标均显著降低,大鼠股骨组织及骨小梁指标得到明显的改善,进一步发现水晶兰苷、巴戟天和E2能够明显升高SOD、GSH-px和CAT的水平,同时能显著降低血清中MDA酶的活性。结论水晶兰苷对绝经后骨质疏松模型大鼠有较好的改善作用,其作用效果与巴戟天、E2阳性药相似,可能是巴戟天抗骨质疏松的主要活性成分。

Objective To investigate the effect of monotropein,an extract of Morinda officinalis on postmenopausal osteoporosis model.Methods SD rats were randomly divided into normal group,model group,E2 group,monotropein group and Morinda officinalis group.The postmenopausal osteoporosis model was established by bilateral gonadectomy,and the administration groups were given monotropein and Morinda officinalis by intragastric administration.After 12 weeks of administration,the rats were dissected,and the serum biochemical indices,femoral morphology and related indices were determined.Results Compared with normal group,serum levels of calcium,phosphorus,alkaline phosphatase,osteocalcin and osteogenesis specific transcription factors were increased,the number of bone trabecular,bone volume fraction,bone surface area and bone volume ratio,bone trabecular thickness were decreased,and the separation degree of bone trabecular was increased in model group.However,after treatment with monotropein and Morinda oficinalis in model rats,the serum biochemical indices(such as Ca,P,ALP,BGP and Runx2)were significantly increased as compared to the model group,and the indexes of femoral tissue and bone trabeculae in rats were significantly improved.It was further found that cymbidine and Morinda officinalis could significantly increase the levels of SOD,GSH-px and CAT,and significantly reduce the activity of MDA enzyme in serum.Conclusion Monotropein has a good effect on improving postmenopausal osteoporosis model,and its effect is similar to that of Morinda oficinalis,which may be the main active ingredient of Morinda officinalis in anti-osteoporosis.