基于UPLC特征图谱和指标成分定量的百蕊草电子束辐照前后品质影响研究

Before and After Electron Beam Irradiation of Thesii Herba Based on UPLC Characteristic Chromatogram and Quantitative Index Components

目的研究电子束辐照灭菌对三个产地百蕊草的特征图谱与各指标成分的影响,为评价辐照百蕊草质量提供依据。方法以百蕊草中5-O-阿魏酰奎宁酸、4-O-阿魏酰奎宁酸,百蕊草素Ⅰ和山柰酚-3-O-(2″-O-α-鼠李糖基-6″-O-丙二酰基)-β-葡萄糖苷的含量作为评价指标,通过指标成分含量和特征图谱的检测,同时联合主成分分析(PCA)与聚类分析(CA),评价不同辐照剂量(1、3、6、10、15、20 kGy)对百蕊草质量的影响。结果不同辐照剂量的百蕊草的特征图谱标定5个共有峰,对比辐照样品与未辐照样品的相似度均大于0.99,PCA与CA结果显示各辐照剂量样品的特征峰面积无明显差异,不同辐照剂量的百蕊草药材整体质量相对稳定;含量测定结果显示,百蕊草辐照后,各指标成分含量有所减少但与辐照剂量并无太大相关性,整体波动范围较小,5-O-阿魏酰奎宁酸与4-O-阿魏酰奎宁酸两个酚酸类成分随着辐照剂量的增大呈下降趋势,是受辐照影响较大的活性成分。结论经电子束不同剂量辐照后的百蕊草的特征图谱并无明显变化,各指标成分含量略有降低,总体变化在可接受范围内,辐照灭菌在研究辐照剂量范围内均适用于三个产地百蕊草药材的灭菌。

Objective e To explore the effects of electron beam irradiation sterilization on the characteristic spectra and various index components of Thesi Herba from three different regions,and provide a basis for evaluating the quality of Irradiated Thesi Herba.Methods With the content of 5-O-ferulic quinic acid,4-O-ferulic quinic acid,thymosin I and kaempferol 3-O-(2″-O-α-rhamsyl-6″-O-malonyl)-β-glucoside as the evaluation indexes.The influence of electron beam irradiation with different irradiation doses(1,3,6,10,15,20 kGy)on the quality of Thesi Herba was evaluated through the detection of the index component content and characteristic spectra,combined with principal component analysis(PCA)and cluster analysis(CA).Results 5 common peaks were calibrated in the characteristic spectra of different irradiation doses of Thesi Herba.The similarity between the irradiated and non-irradiated samples was greater than 0.99,and the PCA and CA results showed that there was no significant difference in the characteristic peak area of each irradiation dose sample.The overall quality of Thesi Herba at different irradiation doses was relatively stable.The content determination results showed that after irradiation,the content of various indicator components in Thesi Herba decreased,but there was no significant correlation with the irradiation dose.The overall fluctuation range was small,especially for the phenolic acid components 5-O-ferulic quinic acid and 4-O-ferulic quinic acid,which showed a decreasing trend with the increase of irradiation dose and were the active components greatly affected by irradiation.Conclusion After being irradiated with different doses of electron beam,there is no significant change in the characteristic spectra of Thesi Herba.The content of each indicator component slightly decreased,and the overall reduction rate is within an acceptable range.Radiation sterilization is applicable to the sterilization of Thesi Herba medicinal materials from 3 different regions within the range of irradiation doses studied.