OPA柱前衍生高效液相色谱法同时测定黄豆酱中氨基酸和生物胺的含量

Determination of Amino Acids and Biogenic Amines in Soybean Paste by Pre-Column Derivatization High Performance Liquid Chromatography

本文采用OPA衍生黄豆酱中的组氨酸、酪氨酸、组胺和酪胺,再通过高效液相色谱进行分离测定。结果表明,该方法的相对标准偏差在0.04%~1.16%,回收率在93.4%~101.2%,检出限为6~9 mg·kg^(-1)。该方法能够满足黄豆酱组氨酸、酪氨酸、组胺和酪胺的检测需求。

In this article,histidine,tyrosine,histamine,and tyramine in soybean paste were derivatized using OPA,followed by separation and determination using high-performance liquid chromatography.The results showed that the RSD of the method was 0.04%~1.16%,the recovery was 93.4%~101.2%,and the detection limit was 6~9 mg·kg^(-1).The method can meet the requirements of detection of histidine,tyrosine,histamine and tyramine during soybean paste fermentation.