毛菊苣乙酸乙酯萃取物对大鼠溃疡性结肠炎合并急性肝损伤防治作用研究

Study on the preventive and therapeutic effects of ethyl acetate extract fromchicory on ulcerative colitis complicated with acute liver injury in Rats

目的研究毛菊苣根乙酸乙酯萃取物(CGEA)对大鼠急性肝损伤合并溃疡性结肠炎的保护作用。方法将SD大鼠36只随机分为正常组(Control)、模型组(Model)、柳氮磺吡啶肠溶片阳性对照组(Sulfasalazine,100 mg·kg^(-1))和CGEA高、低剂量组(150、100 mg·kg^(-1)),每组6只,除正常组外其余各组给予30%TNBS-40%乙醇溶液灌肠(60 mg·kg^(-1)),同时皮下注射含体积分数40%CCl4的橄榄油溶液2 mL·kg^(-1),一周2次,制备急性肝损伤模型合并溃疡性结肠炎模型,造模成功后将CGEA制备成水混悬液,按照CGEA高剂量组(150 mg·kg^(-1)),CGEA低剂量组(100 mg·kg^(-1))连续灌胃大鼠14 d,每日1次。14 d后,处死大鼠并取血清及肝脾组织,计算肝脾指数,检测大鼠血清相关指标;采用HE染色法观察肝、肠组织病理变化。结果与正常组比较,模型组大鼠肝脾指数明显上升(P<0.01);血清中AST、ALT、AKP、γ-GT和MDA水平显著升高(P<0.05或P<0.01),SOD水平显著降低(P<0.05);肝组织遭到破坏,炎性细胞浸润;结肠组织结构杂乱,出现炎症细胞浸润现象。与模型组比较,给药组肝脾指数显著降低(P<0.01);血清中AST、ALT、AKP、γ-GT和MDA指标水平显著降低(P<0.05或P<0.01);给药组肝肠组织破坏程度减轻,炎性细胞浸润减少。结论CGEA可以减轻大鼠溃疡性结肠炎合并急性肝损伤程度,对其起到保护作用。

Objective To study the protective effect of Cichorium root ethyl acetate extract(CGEA)on acute liver injury complicated with acute colitis in Rats.Methods According to 6 Rats in each group,36 SD Rats were randomly divided into Control group,Model group,sulfasalazine enteric-coated tablet positive control group(100 mg·kg^(-1))and CGEA high and low dose groups(150,100 mg·kg^(-1)),Except the normal group,the other groups were given enema with 30%TNBS-40%ethanol solution(60 mg·kg^(-1))and subcutaneous injection of 2 mL·kg^(-1) olive oil solution containing 40%CCl 4 twice a week to prepare acute liver injury model combined with ulcerative colitis model.After successful modeling,CGEA was prepared into water suspension.According to the high-dose group(150 mg·kg^(-1))and low-dose group(100 mg·kg^(-1)).Fourteen days later,the rats were killed,and the serum and liver and spleen tissues were taken,and the liver and spleen index was calculated,and the serum related indexes of rats were detected.The pathological changes of liver and intestine were observed by HE staining.Results The index of liver and spleen in model group was significantly higher than that in normal group(P<0.01).The levels of AST,ALT,AKP,γ-GT and MDA in serum were also higher than those in normal group(P<0.05 or P<0.01)and the level of SOD decreased(P<0.05).Liver tissue was destroyed and inflammatory cells infiltrated;Colon tissue structure is disordered,and microvilli are lost and inflammatory cells are infiltrated.Compared with the model group,the index of liver and spleen in the medication group decreased significantly(P<0.01).Serum levels of AST,ALT,AKP,γ-GT and MDA decreased significantly(P<0.05 or P<0.01).The damage degree of liver and intestine tissue and the infiltration of inflammatory cells were reduced in the treatment group.Conclusion CGEA can alleviate the degree of acute liver injury complicated with ulcerative colitis in rats and protect it.