祛痘方提取物抗炎祛痘体外功效评价

In vitro evaluation of the anti-inflammatory and anti-acne effects of Qudou formulae extract

目的:体外评价祛痘方提取物的表皮屏障修复、抗炎、控油和抗菌功效。方法:分别通过检测对十二烷基硫酸钠(sodium dodecyl sulfate,SDS)诱导的HaCaT细胞损伤模型增殖的影响、对脂多糖诱导的RAW 264.7细胞一氧化氮生成的影响、对SZ95细胞增殖及其中性脂质合成的影响、对金黄色葡萄球菌和痤疮丙酸杆菌的抑制作用来评价祛痘方提取物的表皮屏障修复、抗炎、控油和抗菌功效。结果:祛痘方提取物能够提高SDS诱导的HaCaT细胞的存活率,抑制经脂多糖处理的RAW 264.7细胞分泌一氧化氮,促进SZ95细胞生长并抑制其中性脂质合成,抑制金黄色葡萄球菌和痤疮丙酸杆菌的活性。结论:祛痘方提取物具有显著的表皮屏障修复、抗炎、控油和抑菌功效,有望成为一种新的中药祛痘护肤品原料。

Objective:To explore the effects of Qudou formulae(QF)extract on the epidermal barrier repair,antiinflammatory,sebum control and antibacterial function.Methods:The epidermal barrier repair,anti-inflammatory,sebum control and antibacterial effects of QF extract were evaluated by detecting its effects on sodium dodecyl sulfate(SDS)-induced proliferation of HaCaT cell damage model,lipopolysaccharide(LPS)-induced nitric oxide(NO)production in RAW 264.7 cells,proliferation of SZ95 cells and neutral lipid synthesis,and inhibition of Staphylococcus aureus(S.aureus)and Propionibacterium acnes(P.acnes).Results:QF extract could improve the survival rate of SDS-induced HaCaT cells,inhibit the secretion of NO from LPS-treated RAW 264.7 cells,promote the growth of SZ95 cells and inhibit its neutral lipid synthesis,and had the inhibitory activity against S.aureus and P.acnes.Conclusion:QF extract has significant epidermal barrier repair,anti-inflammatory,oil control and antibacterial effects,and is expected to become a new raw material for Chinese medicine acne skin care products.