利用和频振动光谱探索蛋白质与多肽界面结构的最新研究进展

Recent Advances in Analyzing Protein and Peptide Structures at Interfaces Using Vibrational Sum-Frequency Generation

蛋白质和多肽的界面结构在各种生物过程和技术发展中发挥着重要作用。对这些结构的深入了解对于疾病诊断、药物输送和生物材料的研究至关重要.然而,这些体系的复杂性以及分析方法的局限性限制了其深入研究.尽管X射线晶体学和低温电子显微镜等先进技术在分析蛋白质结构方面做出了重大贡献,但对于实际条件下表面结合蛋白质结构的理解仍然有限,这也为该领域带来了挑战。和频振动光谱技术已经成为分析蛋白质界面分子结构的一种强有力的手段.本文简要介绍和频振动光谱学的基本原理,探讨其在相位测量方面的研究进展,并概述了近三年(2021~2023)的研究成果,展示了和频振动光谱在揭示多肽和蛋白质界面分子结构方面的潜力:本文为进一步利用和频振动光谱探索不同类型的多肽和蛋白质在界面上的结构和功能提供了有利的支持。

The structure of protein and peptide at interfaces plays a crucial role in various biological processes and technological advancements.Understanding these structures is critical for diagnosing diseases,drug delivery,and developing biomaterials.However,the complexity of these systems and limitations in analytical tools have hindered the in-depth exploration.Despite significant efforts in determining protein structures using advanced techniques like X-ray crystallography and cryo-electron microscopy,the understanding of surface-bound protein structures in real conditions remains relatively limited,posing a current challenge in this field.Vibrational sum frequency generation(SFG)spectroscopy has been developed as a versatile method for elucidating molecular structures of proteins across interfaces.This review is intended to introduce the basic principle of SFG spectroscopy,discuss its current advancements in phase measurement,and showcase recent examples(2021–2023)illustrating SFG’s ability in revealing the molecular structure of peptides and proteins at interfaces.This concise review aims to establish a foundation for future studies and applications exploring different types of peptides and proteins at interfaces using SFG.