新型导电填料加速形成厌氧氨氧化生物膜及脱氮效能研究

Accelerate Formation of Anammox Biofilm Using New Conductive Fillers and Its Nitrogen Removal Efficiency

厌氧氨氧化菌(anaerobic ammonia oxidizing bacteria,AnAOB)在实际脱氮工艺中持留能力差,是制约该技术规模化应用的主要技术瓶颈之一。相较于传统生物填料,高温热解轮胎制备成具有导电特性的新型生物填料,不仅可以回收利用固定废弃物,而且能够实现加速AnAOB形成生物膜和提高脱氮效率的双重作用。实验结果表明,800℃最适合用于热解轮胎填料,同时具备更高的电导率和更有利于固定微生物生长特性。相较于单独Anammox组,Anammox+导电填料的NH+4-N、NO-2-N和TN平均去除率比单独Anammox组分别提升了9.12%、13.20%和15.69%,且明显高于聚丙烯(polypropylene,PP)填料的强化效果。Q-PCR分析结果表明,添加导电填料能够促使脱氮菌种丰度增加,其中AnAOB、亚硝酸盐还原菌和硝酸盐还原菌分别增长了50.21%、69.70%和145.59%。该新型导电填料技术的研发,可为Anammox技术推广提供新的思路和数据基础。

Anaerobic ammonia oxidizing bacteria(AnAOB)have poor retention capacity in actual denitrification processes,which is one of the main technical bottlenecks restricting the large-scale application of this technology.Compared with traditional biological fillers,high-temperature pyrolysis tires can be used to prepare a new type of biological filler with conductive properties.This not only recycles fixed waste,but also achieves a dual effect of accelerating the formation of biofilm in AnAOB and improving denitrification efficiency.The experimental results indicate that 800℃is the most suitable for pyrolysis tire fillers,while having higher electrical conductivity and more favorable characteristics for fixed microbial growth.Compared to the Anammox group alone,the average removal rates of NH+4-N,NO-2-N,and TN with Anammox+conductive filler increased by 9.12%,13.20%,and 15.69%,respectively,compared to the Anammox group alone,and were significantly higher than the strengthening effect of polypropylene(PP)filler.The Q-PCR analysis results showed that adding conductive fillers can promote an increase in the abundance of denitrifying bacteria,with AnAOB,nitrite reducing bacteria,and nitrate reducing bacteria increasing by 50.21%,69.70%,and 145.59%,respectively.The development of this new conductive filler technology can provide new ideas and data basis for the promotion of Anammox technology.