三叶因子家族肽3在慢性鼻窦炎伴鼻息肉中的表达及其对黏蛋白5AC表达的调控研究

The expression of TFF3 in chronic sinusitis with nasal polyps and its regulation on MUC5AC expression

目的探究三叶因子家族肽3(trefoil factor family peptide,TFF3)在慢性鼻窦炎伴鼻息肉(CRSwNP)中的表达及其对黏蛋白5AC(MUC5AC)表达的调控。方法选取16例CRSwNP组患者的鼻息肉组织及16例对照组患者的鼻黏膜组织,通过实时荧光定量聚合酶链式反应(RT-qPCR)、Western blot检测TFF3和MUC5AC的表达并进行两者的相关性分析;构建TFF3敲低(KD-TFF3)的人鼻黏膜上皮细胞(HNEpC)系,通过RT-qPCR、免疫荧光技术检测KD-TFF3 HNEpC中MUC5AC的表达。结果TFF3表达水平在鼻息肉组织中较对照组显著降低,MUC5AC表达水平升高,且两者的表达水平具有负相关关系(r=-0.556,P<0.05)。KD-TFF3 HNEpC中,MUC5AC表达较对照组显著升高。结论TFF3在CRSwNP中表达下降且与MUC5AC的表达负相关,为治疗以CRSwNP为代表的慢性鼻腔炎症性疾病黏液异常高分泌提供新的思路。

OBJECTIVE To explore the expression of trefoil factor family peptide(TFF3)in chronic rhinosinusitis with nasal polyps(CRSwNP)and its regulation on mucin 5AC(MUC5AC)expression.METHODS The nasal polyp tissues of 16 patients in the CRSwNP group and the nasal mucosal tissues of 16 patients in the control group were selected,and the expressions of TFF3 and MUC5AC were detected by quantitative real-time polymerase chain reaction(RT-qPCR)and Western blot,and the correlation between them was analyzed.The human nasal epithelial cell(HNEpC)line with TFF3 knockdown was constructed,and the expression of MUC5AC in KD-TFF3 HNEpC was detected by RT-qPCR and immunofluorescence.RESULTS The expression level of TFF3 in nasal polyps was significantly lower than that of control group,and the expression level of MUC5AC was increased,and the expression level of both was negatively correlated(r=-0.556,P<0.05).The expression of MUC5AC in KD-TFF3 HNEpC was significantly higher than that in control group.CONCLUSION The expression of TFF3 decreases in CRSwNP and negatively regulates the expression of MUC5AC.This study provides a new idea for the treatment of abnormal hypersecretion of mucous in chronic nasal inflammatory diseases represented by CRSwNP.